Thermo 线粒体分离试剂盒 货号89874

Thermo 线粒体分离试剂盒  货号89874 
Mitochondria Isolation Kit for Cultured Cells

描述

The Thermo Scientific Mitochondria Isolation Kit for Cultured Cells provides a versatile, microcentrifuge-tube method for fractionating intact mitochondria from cultured mammalian cell samples in about 40 minutes.

Features of the Mitochondria Isolation Kit for Cultured Cells:

• Fast—isolate intact mitochondria from 20 million cells in approximately 40 minutes (post-cell harvest)
• Flexible—process several samples simultaneously using the reagent-based method, or obtain highest possible yield with the Dounce homogenization method
• Optimizable—guidelines provided for optimizing purity vs. yield with the reagent-based method
• Benchtop-easy—isolation performed in a microcentrifuge tube
• Compatible—isolated mitochondria may be lysed with detergent or processed for almost any downstream application, including 2D electrophoresis

Isolation of mitochondria is typically a laborious process requiring single-sample processing with Dounce homogenization. The Mitochondria Isolation Kit uses a non-mechanical, reagent-based method that allows multiple (six) cultured cell samples to be processed concurrently in about 40 minutes. Cultured mammalian cell pellets are gently lysed using a proprietary formulation that results in maximum yield of mitochondria with minimal damage to integrity.

The product instructions describe guidelines for optimizing purity vs. yield parameters. The kit also offers an optimized Dounce homogenization procedure, which results in two-fold greater mitochondria recovery compared to the reagent-based method. Both methods use differential centrifugation to separate the mitochondrial and cytosolic fractions with a benchtop microcentrifuge and are completed in approximately 40 minutes (post-cell harvest). Once isolated, the mitochondria can be used in downstream applications such as apoptosis, signal transduction and metabolic studies, as well as to facilitate mitochondrial proteomics efforts.

Related Products
Mitochondria Isolation Kit for Tissue
Lysosome Enrichment Kit for Tissues and Cultured Cells

规格

Reagent Type:	Organelle Isolation, Subcellular Fractionation
Form:	Kit, Liquid
Product Size:	115 mL

内容及储存

Upon receipt store kit at 4°C. Product is shipped at ambient temperature.

文獻引用及參考文獻

Abcb10 physically interacts with mitoferrin-1 (Slc25a37) to enhance its stability and function in the erythroid mitochondria.
Chen W, Paradkar PN, Li L, Pierce EL, Langer NB, Takahashi-Makise N, Hyde BB, Shirihai OS, Ward DM, Kaplan J, Paw BH
Proc Natl Acad Sci U S A 2009; (106):38 16263-16268

---

Circulating mitochondrial DAMPs cause inflammatory responses to injury.
Zhang Q, Raoof M, Chen Y, Sumi Y, Sursal T, Junger W, Brohi K, Itagaki K, Hauser CJ
Nature 2010; (464):7285 104-107

---

Tubular network formation protects mitochondria from autophagosomal degradation during nutrient starvation.
Rambold AS, Kostelecky B, Elia N, Lippincott-Schwartz J
Proc Natl Acad Sci U S A 2011; (108):25 10190-10195