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- 文献和实验
- 技术资料
- 保存条件:
-20
- 保质期:
3个月
- 英文名:
MiaoLingBio
- 库存:
1000
- 供应商:
淼灵生物
- 规格:
1管
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文献和实验Two-hybrid analysis of genetic
).The most commonly used prey plasmid,pJG4-5,expresses proteins fused to the B42 activation domain,the SV40 nuclear localization signal,and an epitope tag derived from hemagglutinin,all driven by the yeast GAL1 promoter which is active only in yeast grown on galactose
mmol/L YNB 1.34% Biotin (4×10 -5 )% Glycerol 1% 毕赤酵母诱导表达前培养基,YNB和Biotin过滤除菌。培养24小时后,一般静置过夜,待酵母沉淀后倒掉BMGY培养基,改换BMMY培养基,进入诱导表达阶段。 2.5 BMMY培养基 Yeast extract 1% Peptone 2% 磷酸钾缓冲液(pH6.0)100mmol/L YNB 1.34
EXAMINING THE FUNCTION OF PROTEINS AND PROTEIN NETWORKS WITH THE YEAST TWO-HYBRID SYSTEM
. In the most commonly used bait plasmid, pEG202, the bait is expressed from the constitutive yeast ADH1 promoter. Related bait plasmids are available that express the bait fused to a nuclear localization signal. The most commonly used prey plasmid, pJG4-5, expresses
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