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- 文献和实验
- 技术资料
- 保存条件:
-20
- 保质期:
3个月
- 英文名:
MiaoLingBio
- 库存:
1000
- 供应商:
淼灵生物
- 规格:
1管
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文献和实验of 7.5 M of KOAc** to each well, using the Eppendorf repeater pipette. Freeze the tubeds in -70 degrees C for at least two hours to overnight. Thaw. Centrifuge at 13000 rpm for 30 mins. in a microfuge. Transfer ~700 μl of the supernatant
7.Precipitate the DNA using EtOH-OAc and wash with 70% EtOH. 8.Dissolve the DNA in 6 μl dd-water and use the entire solution for ligation and electroporation into pUC18 using the standard protocol. Notes: **7.5 M KOAc - 736.13 gm/liter
.A., Nevill, T., & Ionescu-Zanetti, C. Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells. J. Vis. Exp. (32), e1644, DOI: 10.3791/1644 (2009). 13. Conant, C.G., Schwartz, M.A., & Ionescu-Zanetti, C. Well plate-coupled
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