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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
Influenza B- Virus Wisconsin 01/2010 Recombinant
- 保质期:
1年
- 供应商:
上海沪震实业有限公司
- 保存条件:
-20°C
- 规格:
2μg/10μg/100μg
CATALOGUE NUMBER
IHA-047
INTRODUCTION
Influenza-B virus is a genus in the virusfamily Orthomyxoviridae. The only species in this genus is called "Influenza B virus". Influenza B virus only infects humansand seals. This limited host range is apparently in contrast with those caused by the similar Influenza virus Aas both mutate by both genetic drift and reassortment. Influenza-B virus evolves slower than A viruses and faster than C viruses. Influenza-B virus mutates at a rate 2-3 times lower than type A. However, influenza B mutates enough that lasting immunity is not possible. The Influenza B virus capsidis enveloped while its virionconsists of a matrix protein + envelope + nucleoprotein complex + nucleocapsid, and a polymerasecomplex. Influenza B is sometimes spherical and sometimes filamentous. Its 500 or so surface projections are made of hemagglutinin and neuraminidase.The Influenza B virus is 14648 nucleotideslong and consists of eight segments of linear negative-sense, single-stranded RNA. The multipartite genome is encapsidated, each segment in a separate nucleocapsid, and the nucleocapsids are surrounded by one envelope.
DESCRIPTION
Recombinant Full-Length B/ Wisconsin 01/2010 is glycosylated with N-linked sugars, produced using baculovirus vectors in insect cells.SOURCE
Baculovirus Insect Cells.PHYSICAL APPEARANCE
Sterile Filtered colorless solution.FORMULATION
The Recombinant B/ Wisconsin 01/2010 solution contains 10mM Sodium phosphate, pH 7.1,150mM NaCl and 0.005% Tween-20.STABILITY
B/ Wisconsin 01/2010 recombinant should be stored at 4°C.Do not freeze!
PURITY
Greater than 90.0% as determined by SDS-PAGE.USAGE
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文献和实验Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
) GST‐3C (prepared in‐house) or PreScission 3C protease (GE Healthcare Life Sciences, cat. no. 27‐0843‐01) Histone H1 (Sigma
Culturing BG01V Human Embryonic Stem Cells with Mouse Embryonic Fibroblast (MEF)-Conditioned Media
the pellet. Resuspend the pellet in an appropriate amount of Conditioned Media supplemented with 4 ng/mL of recombinant human FGF basic. Add the BG01V hES cell suspension to the Cultrex BME-coated plate. Grow in a 37° C, 5% CO2
Procedure for Culturing BG01V Human Embryonic Stem Cells with Human Feeder Cell Conditioned Medium
Recombinant human FGF basic (R&D Systems, Catalog # 233-FB , 4114-TC , or equivalent) Accutase (Innovative Cell Technologies, Catalog # AT104 or equivalent) Cultrex® Basement Membrane Extract (BME) (R&D Systems, Catalog # 3433-005-01 or equivalent
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