FITC标记小鼠抗人CD34单克隆抗体

英文名称human CD34-FITC
中文名称FITC标记小鼠抗人CD34单克隆抗体
别    名CD34 antigen; CD34 molecule; Cluster designation 34; Hematopoietic progenitor cell antigen CD34; HPCA1; CD34_HUMAN.  FITC anti-human CD34;
规格25T  100T  
研究领域肿瘤  心血管  免疫学  神经生物学  干细胞  细胞表面分子  细胞类型标志物  血管内皮细胞  内皮细胞  
抗体来源Mouse
克隆类型Monoclonal
交叉反应 Human, 
产品应用Flow-Cyt=20ul/Test （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量39kDa
细胞定位细胞膜 
性    状Liquid
免 疫 原: 
亚    型IgG
纯化方法affinity purified by Protein G
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
FITC标记小鼠抗人CD34单克隆抗体产品介绍background:
The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.

Function:
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Tissue Specificity:
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

Post-translational modifications:
Highly glycosylated. 
Phosphorylated on serine residues by PKC.

Similarity:
Belongs to the CD34 family.

SWISS:
P28906

Gene ID:
947

Database links:
Entrez Gene: 947 Human
Omim: 142230 Human
SwissProt: P28906 Human
Unigene: 374990 Human


Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

FITC标记小鼠抗人CD34单克隆抗体产品图片

产品图片	Flow cytometry staining of normal human peripheral blood cells with CD34/FITC (bsm-30079M-FITC)(filled histogram) or Mouse IgG1 Isotype Control (FITC Conjugate)(C03-11001)(open histogram). Total cells were used for analysis.