APC标记小鼠抗人CD33单克隆抗体

英文名称human CD33-APC
中文名称APC标记小鼠抗人CD33单克隆抗体
别    名CD33_HUMAN; CD 33; CD33 antigen; CD33 molecule; FLJ00391; gp67 ; Myeloid cell surface antigen CD33; Myeloid cell surface antigen CD33 precursor; p67 ; Sialic acid binding Ig like lectin 3; Sialic acid binding immunoglobulin like lectin 3; SIGLEC3; Siglec-3; myeloid cell surface antigen CD33 isoform 1 precursor.  APC anti-human CD33;
规格25T  100T  
研究领域免疫学  干细胞  细胞表面分子  
抗体来源Mouse
克隆类型Monoclonal
交叉反应 Human, 
产品应用Flow-Cyt=20ul/Test （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量40/37kDa
细胞定位细胞膜 
性    状 Liquid
免 疫 原: 
亚    型IgG
纯化方法affinity purified by Protein G
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
APC标记小鼠抗人CD33单克隆抗体产品介绍background:
CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.

Function:
Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).

Subunit:
Interacts with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein.

Tissue Specificity:
Monocytic/myeloid lineage cells.

Post-translational modifications:
Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11.
Phosphorylation of Tyr-358 is involved in binding to PTPN6.

Similarity:
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. 
Contains 1 Ig-like V-type (immunoglobulin-like) domain.

SWISS:
P20138

Gene ID:
945

Database links:
Entrez Gene: 945 Human
Omim: 159590 Human
SwissProt: P20138 Human
Unigene: 83731 Human


Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

APC标记小鼠抗人CD33单克隆抗体产品图片

Flow cytometry staining of normal human peripheral blood cells with CD33/APC(bsm-30078M-APC)(Red histogram) or Mouse IgG2a Isotype Control (APC Conjugate)(C03-12004)(Blue histogram) . Total cells were used for analysis.