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- 文献和实验
- 技术资料
- 克隆性:
多克隆
- 抗体英文名:
mouse IgG1-FITC Isotype Control
- 规格:
50T
英文名称mouse IgG1-FITC Isotype Control
中文名称FITC标记小鼠IgG1同型对照
别 名FITC mouse IgG1; mouse IgG1/FITC; mouse IgG1 FITC conjugated;
规格50T
研究领域
抗体来源Rabbit
克隆类型Polyclonal
交叉反应
产品应用(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状Liquid
免 疫 原:
亚 型IgG1
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
中文名称FITC标记小鼠IgG1同型对照
别 名FITC mouse IgG1; mouse IgG1/FITC; mouse IgG1 FITC conjugated;
规格50T
研究领域
抗体来源Rabbit
克隆类型Polyclonal
交叉反应
产品应用(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状Liquid
免 疫 原:
亚 型IgG1
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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文献和实验相关实验
当FITC在碱性溶液中与抗体蛋白反应时,主要是蛋白质上赖氨酸的r氨基与荧光素的硫碳胺键(thiocarbmide)结合,形成FITC-蛋白质结合物,即荧光抗体或荧光结合物。一个IgG分子中有86个赖氨酸残基,一般最多能结合15~20个,一个IgG分子可结合2~8个分子的FITC,其反应式如下FITC-N=C=S + N-H2-蛋白质 → FITC-NS-C-N-H2-蛋白质常用Marsshall(1958)法标记荧光抗体,也可以根据条件采用Chadwick等标记法或Clark
ml三蒸水中即成; 方法与步骤: 根据Marshall氏法高效价的抗人球蛋白兔免疫血清,分离球蛋白。 1. 用0.15 mol/L NaCl的盐水及0.15 mol/L pH9.0的NaHCO3-Na2CO3缓冲液稀释使每毫升内含抗体10mg,缓冲液为总量的10%; 2. 将以上溶液降温至4℃,按蛋白:荧光素=50—80mg:1mg的比例加入异硫氰酸荧光素,在0—4℃下电磁搅拌12—14h; 3.用半饱和硫酸铵将标记球蛋白
5. 过柱。取透析过夜的标记物,过葡萄糖凝胶G-25或G-50柱,分离出游离荧光素,收集标记的荧光抗体进行鉴定。
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FITC标记小鼠IgG1同型对照
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