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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
CD58, Mouse Anti-Human
- 保质期:
1年
- 供应商:
上海沪震实业有限公司
- 保存条件:
-20°C
- 规格:
500µg/1mg
CATALOGUE NUMBER
ANT-277
SYNONYMS
CD58, LFA-3, Ag3, Surface glycoprotein LFA-3.TYPE
Mouse Anti Human Monoclonal.INTRODUCTION
CD58 is part of the immunoglobulin superfamily. CD58 is a ligand of the T lymphocyte CD2 protein, and is invloved in adhesion and activation of T lymphocytes. CD58 is localized to the plasma membrane.CLONE
hCD58.IMMUNOGEN
Human Erythrocytes.IG SUBCLASS
Mouse IgG1.PURIFICATION METHOD
Ion-Exchange Column.FORMULATION
1mg/ml in PBS (after reconstitution).STORAGE PROCEDURES
Lyophilized: store at 4°C. After reconstitution, if not intended for use within a month, aliquot and store at -20°C.SOLUBILITY
Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.APPLICATIONS
Staining antibody. For staining, use 10µl/1,000,000 cells.AVAILABLE CONJUGATES
This antibody is also available conjugated to biotin and FITC. For staining with biotin or FITC-conjugated antibody use 5-10µl/1,000,000 cells.USAGE
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验CD58 分子 CD58 常用单克隆抗体或代号: G26,BRIC5;(LFA- 3) 主要表达细胞: 广泛 [AS] 分子质量(kDa)和结构: gp55- 70,(部分GPI连接)(IgSF) 功 能: 与CD2结合,粘附 CD58 Aka LFA-3 (lymphocyte function associated antigen); ligand for CD2
Native purification of His-tagged Proteins from Sf9 cells
Protocol is for 150 ml of cell culture. 1. Spin down cells and wash 1X in 10 ml ice cold PBS. 2. Resuspend pellet in 20 ml lysis buffer: 50 mM Tris pH 8.0 1 % NP-40 1 mM PMSF 1 µg/ml leupeptin 3. Transfer to oakridge tubes and sonicate
GPCR Expression Using Baculovirus-Infected Sf9 Cells
Expression of proteins in insect cells using recombinant baculoviruses has gained wide use in the G protein-coupled receptor (GPCR) community. This expression system produces high yields of functional receptor, is able to perform post
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