Dopamine Receptor D1多巴胺受体D1抗体

英文名称Dopamine Receptor D1
中文名称多巴胺受体D1抗体
别    名DRD1; dopamine D1 receptor; D(1A) dopamine receptor; D1A dopamine receptor; Dopamine D1Receptors; D1DR; DADR; Dopamine Receptor D1; DR D1; DR D1A; DRD 1; DRD1 receptor; DRD1; DRD1A; DRD1_HUMAN; D(1A) dopamine receptor; DRD 1A; DRD1.  
文献引用


规格50ul  100ul  200ul
研究领域肿瘤  免疫学  神经生物学  
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, 
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量50kDa
细胞定位细胞浆 细胞膜 
性    状Lyophilized or Liquid
浓    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human DRD1:101-200/446 <Extracellular>
亚    型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. [provided by RefSeq, Jul 2008]

Function:
Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.

Subunit:
Interacts with DNAJC14 via its C-terminus. Interacts with DRD1IP.

Subcellular Location:
Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.

Tissue Specificity:
Detected in caudate, nucleus accumbens and in the olfactory tubercle.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
P21728

Gene ID:
1812

Database links:
Entrez Gene: 1812 Human
Entrez Gene: 13488 Mouse
Entrez Gene: 24316 Rat
Omim: 126449 Human
SwissProt: P21728 Human
SwissProt: Q61616 Mouse
SwissProt: P18901 Rat
Unigene: 2624 Human
Unigene: 54161 Mouse
Unigene: 24039 Rat


Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

多巴胺受体D1在运动协调方面起重要作用，该受体的缺失对黑质多巴胺能神经元的影响程度虽没临床帕金森病（PD）严重，但仍可加速多巴胺能神经元发生退行性改变.该蛋白目前主要用于神经退行性改变的研究。

产品图片

Sample: 293T Cell Lysate at 40 ug Primary: Anti- Dopamine Receptor D1 (bs-1007R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 50 kD Observed band size: 48 kD Sample: Cerebrum (Mouse)Lysate at 40 ug Primary: Anti- Dopamine Receptor D1 (bs-1000R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 50 kD Observed band size: 48 kD Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-DRD1 Polyclonal Antibody, Unconjugated(bs-1007R) 1:300, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: HUVEC cells(blue). Primary Antibody:Rabbit Anti-CD31 antibody(bs-0468R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) .Primary antibody (bs-0468R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed Blank control: Hela(blue).  Primary Antibody:Rabbit Anti- Dopamine Receptor D1 antibody(bs-1007R), Dilution: 1レg in 100 レL 1X PBS containing 0.5% BSA;  Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );  Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Antibody (bs-1007R, 1レg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of bs-1007R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.