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文献和实验Transgenic Mouse and Transgenic Rat Outline
into fertilized mouse or rat eggs, the Transgenic Core requires that you provide evidence that you have a PCR or Southern blot assay that detects your transgene when it is spiked into tail DNA at a one copy concentration. A second assay that will detect
Organ Culture of Intact Metanephric Kidneys
, and both the ureter and bladder become enveloped in mesodermal-derived smooth muscle. Fig. 1. Differentiation of the metanephric kidney. ( A ) Histology of a rat metanephric kidney in situ at d 12.5 of gestation (the equivalent
Liposome-Mediated, Fluorescence-Based Studies of Sphingolipid Metabolism in Intact Cells
), and brain lipase (21 –23 ). For assaying these enzymes we labeled the respective substrates with tritium (3 H) or 14 C and developed procedures for separating the hydrolytic products from the, as yet, nondegraded substrates. In the late 1970s we began
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