TNC Human

TNC Human

CATALOGUE NUMBER

PRO-322

SYNONYMS

Troponin C slow skeletal and cardiac muscles, TN-C, TNNC1, TNNC, TNC, CMD1Z.

INTRODUCTION

Troponin is a fundamental regulatory protein of striated muscle contraction, and together with tropomyosin, is positioned on the actin filament. Troponin has 3 subunits: TnI- the inhibitor of actomyosin ATPase; TnT- contains the binding site for tropomyosin; and TnC- the protein encoded by the TNNC1 gene. The binding of calcium to TnC stops the inhibitory action of TnI, consequently allowing the interaction of actin with myosin, the hydrolysis of ATP, and the generation of tension. Mutations in the TNNC1 gene are linked to cardiomyopathy dilated type 1Z.

DESCRIPTION

Recombinant Human TNC produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 18,020 Dalton.
The TNC is purified by proprietary chromatographic techniques.

SOURCE

Escherichia Coli.

PHYSICAL APPEARANCE

Sterile Filtered clear solution.

FORMULATION

The protein solution contains 150mM NaCl, 10mM sodium phosphate, 0.05% NaN3, pH 7.0.

STABILITY

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. 
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

PURITY

Greater than 95.0% as determined by both:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

PROTEIN CONTENT

Protein quantitation was carried out by using 0.25 - 5.0 mg/ml Bradford assay vs. BSA.

USAGE

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.