Ki-67Ki67蛋白抗体

英文名称Ki-67
中文名称Ki67蛋白抗体
别    名Antigen identified by monoclonal antibody Ki 67; Antigen KI67; KIA; Ki-67; Ki67; MKI67; Proliferation related Ki 67 antigen; Antigen KI-67; KI67_HUMAN.  

规格50ul  100ul  200ul
研究领域肿瘤  细胞生物  信号转导  转录调节因子  
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Rabbit, 
产品应用ELISA=1:500-1000 Flow-Cyt=1μg/Test （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量358kDa
细胞定位细胞核 
性    状Lyophilized or Liquid
浓    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human Ki67:1201-1300 
亚    型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Proliferation Marker

Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

Function:
Thought to be required for maintaining cell proliferation.

Subcellular Location:
Nucleus. Chromosome. Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.

Similarity:
Contains 1 FHA domain.

SWISS:
P46013

Gene ID:
4288

Database links:
Entrez Gene: 4288 Human
Entrez Gene: 17345 Mouse
Entrez Gene: 246042 Rat
Omim: 176741 Human
SwissProt: P46013 Human
SwissProt: Q91VE6 Mouse
SwissProt: Q5RJM0 Rat
Unigene: 689823 Human
Unigene: 80976 Human
Unigene: 4078 Mouse
Unigene: 233802 Rat


Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

细胞增殖标志物(Proliferation Marker) 
Ki67与PCNA一样，为细胞增殖的一种标记，在细胞凋亡中S、G2 、M期均有表达，G0期缺如。 
Ki-67增殖指数高低与许多肿瘤的分化程度、浸润、转移以及预后密切相关，因此被广泛作为各种恶性肿瘤的必检项目之一。
产品图片Generously provided by Markus Linder from Medical University Vienna as part of the Bioss Discovery Program. Formalin-fixed, paraffin embedded, and decalcified in EDTA embryonic mouse bone labeled with Anti-Ki-67 (Proliferation Marker) Polyclonal Antibody, Unconjugated (bs-2130R) at 1:100 followed by conjugation to the secondary antibody and DAB staining Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ki-67) Polyclonal Antibody, Unconjugated (bs-2130R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ki-67) Polyclonal Antibody, Unconjugated (bs-2130R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ki-67) Polyclonal Antibody, Unconjugated (bs-2130R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control (blue line): Mouse brain (fixed with 2% paraformaldehyde for 10 min at room temperature, and then stained with Primary Antibody for 30 min at room temperature). 
Primary Antibody (green line): Rabbit Anti-Ki-67 antibody (bs-2130R),Dilution: 1μg /10^6 cells. 
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1μg /test.