Human HDL ELISA Kit

Human HDL ELISA Kit

Catalog No.: IRKTAH2561

$495
Category: Assay and ELISA Kits

Product Description

Human HDL ELISA Kit

Human HDL ELISA Kit is designed for detection of HDL in plasma, serum, milk, and cell culture supernatant. This assay employs a quantitative competitive enzyme immunoassay technique that measures HDL in less than 3 hours. A polyclonal antibody specific for HDL has been pre-coated onto a 96-well microplate with removable strips. HDL in standards and samples is competed with a biotinylated HDL sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

Human high-density lipoprotein (HDL) is the smallest and the densest of the discoidal and spherical lipoprotein particles. When fractionated by ultracentrifugation, HDL is separated into two major sub-fractions HDL2 (d 1.063 1.125 g/ml) and HDL3 (d 1.125 1.21 g/ml) (1 - 2). It contains 70 of apolipoprotein A-I, 20 of apolipoprotein A-II, phospholipids, and free cholesterol. HDL delivers cholesterol to liver cells which then secrete bile acids and cholesterol for excretion or re-utilization (3). HDL plays important anti-atherogenic roles, including cellular cholesterol efflux capacity, antioxidative, anti-inflammatory, antiapoptotic, vasodilatory, antithrombotic, and anti-infectious activities (4). Low plasma HDL cholesterol is an independent risk factor for the development of premature atherosclerosis. A rare form ofgenetic HDL deficiency is Tangier disease which is associated with mutations in the ATP-binding cassette transporter 1 gene (5).

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