AffiniPure Rat Anti-Mouse IgG

Rat x Rat Hybridomas

There is an increasing interest in the preparation of rat � rat hybridomas, because they have been found to be more stable in culture than mouse hybridomas and they secrete consistently high levels (10 �g/mL and above) of monoclonal antibody

E L I S A w i t h p l a t e l e t s

for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS

Jackson ImmunoResearch 二抗选用指南

, 这种抗体在检测亚美尼亚仓鼠IgG 单克隆抗体时，它的效果没有抗亚美尼亚仓鼠IgG 好。 注意：抗亚美尼亚仓鼠IgG(H+L)(min X Bov, Hu, Ms , Rb, Rat Sr Prot) 不能识别所有的亚美尼亚仓鼠单克隆抗体，因为它经相近物种的吸附处理过（粗体字）。因此，使用经过少数物种或少数相近物种的吸附处理过的抗体可达到较好的效果，如抗亚美尼亚仓鼠IgG(H+L)(min X BoV Sr Prot), 除此之外，在小鼠或大鼠免疫球蛋白中也应该检测亚美尼亚仓鼠单抗。