Mouse Anti Dinitrophenyl (DNP

                                                         Cat # DNP14-M Mouse monoclonal Anti-DNP IgE, aff pure SIZE: 100 ug

Asthma is a chronic lung disease characterized by airway hyper-responsiveness (AHR) to allergens, airway edema, and increased mucus secretion. Increased levels of circulating IgE and IgG1 antibody and a propensity to allergic responses, atopy, are associated with the development of asthma. Animal models of AHR, where control of the timing of exposure to the initiating antigen, the use of a defined allergen trigger, and genetic manipulation are likely to enhance the understanding of AHR. Dinitrophenol (DNP) has long been employed as a model immunogen as a single antigen to reduce the complexity of modeling AHR.

One of the primary aspects of the immune response is the interaction of antigens with lymphocytes to induce the formation of antibodies, that in turn makes the antigen harmless. Much of our current understanding of the antibody response to antigens has been derived by using the antibody-hapten model. One known model uses the dinitrophenyl (DNP) group. Immunization of many mammalian species with DNP-protein conjugates results in production of antibodies specific for DNP and the amino acid side chains to which it is attached.
DNP conjugates (DNP-KLH or DNP-BSA) useful for the production of antibodies specific for DNP and hemocyanin or BSA. DNP-immunization produced a significant variation in the amount and antibody class (IgGs, IgA, IgE, IgM) among strains, and under various experimental conditions. DNP preparations (purity and supplier), doses (amount per injection), routes (intramuscular, intravenous, aerosol, liposome entrapped, polymerized etc), frequency of exposure (single injections, multiple etc) may induce a defined class of antibody and its level may vary as well. DNP-induced antibody production has been used to assess the immune status of normal and immune-compromised animals.

Suggested Applications

Western blot: 0.5-5 ug/ml for detecting DNP-modified proteins using ECL. DNP-modified known protein standards are available for controls (cat # DNP45-C).
ELISA: 0.1-1 ug/ml for detecting DNP-modified proteins
Species Specificity
Antibody # DNP14-M reacts with DNP. Antibodies to KLH have largely been removed by affinity purification using DNP-BSA-Agarose affinity purification. DNP-BSA protein conjugate (Cat # DNP35-N-10) is also available to test antibody by ELISA or Western. It may also react with KLH protein from related species. Species crossreactivity is not experimentally confirmed. Anti-KLH antibodies produced in mouse, goat and sheep are also available.