- 询价
- invitrogen
- MA515739
- 2025年08月07日
- ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
- Mouse
- Chicken, Human, Mouse, Rabbit, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
beta Actin Loading Control Monoclonal Antibody (BA3R)
- 靶点：
beta Actin Loading Control
- 浓度：
1 mg/ml
- 应用范围：
ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
- 宿主：
Mouse
- 适应物种：
Chicken, Human, Mouse, Rabbit, Rat
- 克隆性：
Monoclonal
- 保存条件：
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- 形态：
Liquid
- 亚型：
IgG2b
- 免疫原：
Beta-actin N-terminal peptide.
- 规格：
100 µg
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文献和实验Western Blotting with Monoclonal Ant
). 2.Aspirate excess PBS. 3.Add 1ml boiling 2X concentrated electrophoresis sample buffer (125mM Tris pH 6.8, 4% SDS, 10% glycerol, 0.006% bromophenol blue, 1.8% beta-mercaptoethanol). 4.Scrape cells from dish, transfer to a microcentrifuge tube, and boil
Western Blotting with Monoclonal Antibodies
plate rapidly with phosphate-buffered saline (PBS). Aspirate excess PBS. Add 1ml boiling 2X concentrated electrophoresis sample buffer (125mM Tris pH 6.8, 4% SDS, 10% glycerol, 0.006% bromophenol blue, 1.8% beta-mercaptoethanol).
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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