- 询价
- invitrogen
- A15778
- 2025年07月10日
- Flow Cytometry
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
CD300a Monoclonal Antibody (MEM-260), PE
- 靶点：
CD300a
- 应用范围：
Flow Cytometry
- 宿主：
Mouse
- 适应物种：
Human
- 克隆性：
Monoclonal
- 保存条件：
4° C
- 形态：
Liquid
- 亚型：
IgG1
- 免疫原：
HPB human lymphoid leukemia cell line.
- 规格：
100 tests
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文献和实验Monoclonal Antibody Production Protocol
(1)Cell Fusion and Selection Solutions: - IMDM - IMDM complete + 15% MCM. - PBS - PEG 50% ( w/v ): PEG 4000 ( 50% ) from Boehringer Manheim 1 243 268 - Aminopterin: Sigma, A 5159 (50X ) Prepare a T-150 flask with 170ml of IMDM
Chimerization of a Monoclonal Antibody for Treating Hodgkin's Lymphoma
Conventionally, monoclonal antibodies (MAbs) are generated by fusing B cells from an immunized animal with myeloma cells from the same species (1 ). Several murine MAb have already been employed for in vivo diagnosis and therapy, including
Polyclonal and Monoclonal Antibodies
later again (2 + 2 week cycle). Monoclonal Antibody If titre is positive do one of the following 2 weeks later: I - Boost tail vein with 20 ug- 50 ug of Antigen in PBS and proceed with fusion on day 4. OR II - Boost
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