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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Goat anti-Mouse IgG1 Secondary Antibody, PE-Cyanine5
- 靶点:
Mouse IgG1
- 应用范围:
Flow Cytometry
- 宿主:
Goat
- 适应物种:
Mouse
- 克隆性:
Polyclonal
- 保存条件:
4° C, store in dark
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
IgG gamma 1
- 规格:
500 µL
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文献和实验secondary antibody review -- data from 99 publications
Jackson ImmunoResearch Laboratories 11 goat IgG TRITC immunohistochemistry 1:40 detect amelogenin in mouse postnatal mandibular molars Jackson ImmunoResearch Laboratories 12 IgG IgG1 FITC immunocytochemistry 1:1000 Santa Cruz Biotechnology 13
T-cell dependent antigen specific in situ staining (Pe)
-conjugated secondary antibody (Typically SBA goat anti rabbit-AP 1:100, rabbit anti goat-AP 1:300) 22- wash twice 23- [optional] add a tertiary layer (rabbit anti-goat-AP on a goat anti-rabbit-AP) 24- wash thrice 25- develop AP (see protocol
Protocol for anti-HA antibody Western Blotting
seconds, and pour it off: this method was worked out in the Yamamoto lab eons ago). 7) Incubate with secondary antibody (anti-mouse) in 5% BSA/PBS/Tween as you would ordinarily. I usually go for 2 hours @ room temp. 8) 12-15 quick washes again(in 1XPBS
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