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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
CD45 Monoclonal Antibody (HI30), PE
- 靶点:
CD45
- 应用范围:
Flow Cytometry
- 宿主:
Mouse
- 适应物种:
Human
- 克隆性:
Monoclonal
- 保存条件:
4° C, store in dark
- 形态:
Liquid
- 亚型:
IgG1
- 免疫原:
CD45 antigen
- 规格:
2 ml
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Immune Response Biomarker Profiling
a total of 5 wash steps. 8) During the wash steps, mix 2.5 L Alexa Fluor® 647 goat anti-human IgG antibody with 5 mL Washing Buffer per array to obtain a final antibody concentration of 1 g/mL. Store on ice until use. Optional: add Alexa Fluor®®
Combined Flow Cytometric Measurement of Two Cell-Surface Antigens and DNA-RNA Content
If the cell surface protein of interest is expressed at high levels, it may be possible to use a mAb that is directly conjugated to Alexa Fluor 488, omitting the need for indirect staining using a biotinylated antibody. If this is the case, omit
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