- 询价
- invitrogen
- MA135946
- 2025年07月09日
- Flow Cytometry
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
CD229 Monoclonal Antibody (HLy 9.1.25), PE
- 靶点：
CD229
- 应用范围：
Flow Cytometry
- 宿主：
Mouse
- 适应物种：
Human
- 克隆性：
Monoclonal
- 保存条件：
4° C, store in dark
- 形态：
Liquid
- 亚型：
IgG1
- 免疫原：
Mouse pre-B cell line 300.19 transfected with CD229 cDNA.
- 规格：
100 tests
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文献和实验ELISA FOR DETECTING SECRETED ICAM-1
Purpose Materials NUNC-Immuno Plate IIF. Horseradish Peroxidase-Streptavidin (Zymed; 43-4323) PBS or Balanced Salt Solution (BSS). Do not use RPMI or any medium that contains biotin. BSA (1% in PBS). ABTS
SANDWICH ELISA FOR DETECTING SECRETED ICAM-1
and substrate buffer (Zymed; #00-2011) ABTS chromogen solution Hydrogen Peroxidase solution Citrate buffer Anti-ICAM-1 mAb R6.5 or CL203. Use as 10 ug/ml in PBS to coat plates. Biotinylated mAb: Biotin RR
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
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