- 询价
- invitrogen
- MA517858
- 2025年07月06日
- Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
- Rat
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
CD11b Monoclonal Antibody (M1/70.15), Biotin
- 靶点：
CD11b
- 浓度：
0.1 mg/ml
- 应用范围：
Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
- 宿主：
Rat
- 适应物种：
Mouse
- 克隆性：
Monoclonal
- 保存条件：
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- 形态：
Liquid
- 亚型：
IgG2b
- 免疫原：
C57BL/10 spleen cell enriched for T lymphocytes
- 规格：
300 ug
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文献和实验Monoclonal Antibody Production Protocol
(1)Cell Fusion and Selection Solutions: - IMDM - IMDM complete + 15% MCM. - PBS - PEG 50% ( w/v ): PEG 4000 ( 50% ) from Boehringer Manheim 1 243 268 - Aminopterin: Sigma, A 5159 (50X ) Prepare a T-150 flask with 170ml of IMDM
in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
The production of monoclonal antibody by hybridoma
, A., Popkov, M., Cortez, X., Fuller, R., Elia, M., Kerwin, L., and C.F. Barbas III. (2003) Rapid monoclonal antibody generation via dendritic cell targeting in vivo. Hybridoma and Hybridomics 22 (1), 23-31.
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