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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
CD277 Monoclonal Antibody (eBioBT3.1 (20.1, BT3.1)), eBioscience™
- 靶点:
CD277
- 浓度:
0.5 mg/mL
- 应用范围:
Flow Cytometry, Immunoprecipitation
- 宿主:
Mouse
- 适应物种:
Human
- 克隆性:
Monoclonal
- 保存条件:
4° C
- 形态:
Liquid
- 亚型:
IgG1
- 规格:
100 µg
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文献和实验Monoclonal Antibody Production
. (7) Centrifuge samples at 10,000g 10 min. (8) Pipet off the serum supernatant.Store at -20 ℃.This is your pre-bleed control. Hybridoma Fusion 2. For one fusion you will need (1) 8500 ml bottles of DMEM media (2) 3500 ml bottles of fetal calf serum (FCS
membrane, creating a structure similar to an ion channel Delivers early signal in B cell activation, allowing resting B cells to respond to later antigens Rituximab is a chimeric murine-human anti-CD20 antibody used for treatment of B cell
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
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