- 询价
- invitrogen
- PA531817
- 2025年07月13日
- ChIP assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Tri-Methyl-Histone H3 (Lys27) Polyclonal Antibody
- 靶点：
Tri-Methyl-Histone H3 (Lys27)
- 浓度：
0.5 mg/ml
- 应用范围：
ChIP assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
- 宿主：
Rabbit
- 适应物种：
Human
- 标记物：
Methylated: Lys27
- 克隆性：
Polyclonal
- 保存条件：
-20° C, Avoid Freeze/Thaw Cycles
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
Synthetic peptide corresponding to a region within amino acids 22 and 36 of Human Histone H3
- 规格：
100 µL
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文献和实验then serially diluted in 100 µl assay buffer on the plate). Incubate @ RT for 1 hr while shaking. Meanwhile let TMB solution equilibrate to RT. Wash plate 3x and add 100 µl/well rabbit polyclonal anti-p27 antibody diluted 1:1000 in assay buffer
(either undiluted or 1:1 with PBT) or diluted polyclonal antiserum and incubate at 4 C overnight 8. Rinse with PBT 3 times, then wash 4 times for 15 minutes each 9. Add secondary antibody (usually 1:200 in PBT) and incubate 2 hours at room temperature
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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