Pertussis Toxin Mutant百日咳毒素（突变

百日咳杆菌（bordetella pertussis）

百日咳杆菌（ bordetella pertussis ）是人类百日咳的病原菌。百日咳杆菌为卵圆形短小杆菌，大小为 0.5 ～ 1.5 × 0.2 ～ 0.5um ，属鲍特氏菌属 bordetella) ，无鞭毛、芽胞。革兰氏染色阴性。用甲苯胺蓝染色可见两极异染颗粒。专性需氧，初次分离培养时营养要求较高，需用马铃薯血液甘油琼脂培养基（即鲍～金氏培养基）才能生长。经 37 ℃ 2 ～ 3 天培养后，可见细小、圆形、光滑、凸起、银灰色、不透明的菌落，周围有模糊的溶血

ADP-Ribosylation of -Gi Proteins by Pertussis Toxin: Positional Dissection of Acceptor Sites Using Membrane Anchored Synthetic Peptides

Bordetella pertussis , the causative agent of whooping cough, secretes a plethora of virulence factors contributing to the onset and characteristic of the disease. The exotoxin pertussis toxin (PT) is regarded as particularly important

Pyrosequencing在分子诊断方面的应用

4.Boom, R. et al. Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28(3) 495-503 (1990). 二．细菌分型：Bordetella pertussis（百日咳杆菌）和parapertussis（副百日咳杆菌）     百日咳(pertussis,whooping cough)是由百日咳杆菌所致的急性