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96T
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文献和实验Tissue Culture of Skeletal Muscle
obtainable from a wide variety of species, including humans. Moreover, the stem cells from which muscle develops in tissue culture seem to be very resilient; they will survive anoxic conditions within muscle at temperatures between 4 and 37�C
Delivery of DNA into Skeletal Muscle in Large Animals
Increased transgene expression after plasmid transfer to the skeletal muscle is obtained with electroporation in many species, but optimal conditions for individual species and muscle group are not well defined. Using a muscle-specific
AAV-Mediated Gene Transfer to Skeletal Muscle
to nondividing cells such as muscle fibers or hepatocytes following in vivo transduction (1 –7 ). Recombinant AAV can be administered to skeletal muscle of experimental animals and, as recently documented in a Phase I clinical trial, to humans at high vector
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