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- LMAI Bio
- 中国/上海
- LM4899
- 2025年07月05日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pSicoR PGK puro(pSicoRPGKpuro)
- 库存：
大量
- 供应商：
联迈生物
- 规格：
1ug
质粒名称: pSicoR PGK puro
其他名称:
质粒大小（bp）: 7700
抗性: Ampicillin
质粒类型: Mammalian Expression, Lentiviral, RNAi, Cre/Lox
是否病毒:
启动子:
稳转或瞬转:
组成型或诱导性:
表达水平: High
蛋白标签:
测序引物:
GenBank号:
其他属性: Modifications to Backbone: Puromycin is expressed from this plasmid as a selectable marker. Puromycin and the shRNA oligo are flanked by LoxP sites. Cre causes both to be recombined out of the construct, turning off shRNA expression.
Cloning site 5′: HpaI,Site destroyed during cloning:No
Cloning site 3′:XhoI,Site destroyed during cloning:No
5′ sequencing primer:mU6-F List of Sequencing Primers
Growth strain(s): DH5alpha
Growth temperature (℃): 37
Selectable markers:Puromycin
质粒图谱:
质粒序列:
>12084 with CMV-F
CGCGTTTTGCCTGTACTGGGTCTCTCTGGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGG
GAACCCACTGCTTAAGCCTCAATAAAGCTTGCCTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTGTGT
GACTCTGGTAACTAGAGATCCCTCAGACCCTTTTAGTCAGTGTGGAAAATCTCTAGCAGCGTGTCCCCAA
AAG
>12084 with mU6-F
TGTTTTGAGACTATAAATATCCCTTGGAGAAAAGCCTTGTTAACGCGCGGTGACCCTCGAGTACTAGGAT
CCATTAGGCGGCCGAATTCGTCGACCTCGAAATTCTACCGGGTAGGGGAGGCGCTTTTCCCAAGGCAGTC
TGGAGCATGCGCTTTAGCAGCCCCGCTGGGCACTTGGCGCTACACAAGTGGCCTCTGGCCTCGCACACAT
TCCACATCCACCGGTAGGCGCCAACCGGCTCCGTTCTTTGGTGGCCCCTTCGCGCCACCTTCTACTCCTC
CCCTAGTCAGGAAGTTCCCCCCCGCCCCGCAGCTCGCGTCGTGCAGGACGTGACAAATGGAAGTAGCACG
TCTCACTAGTCTCGTGCAGATGGACAGCACCGCTGAGCAATGGAAGCGGGTAGGCCTTTGGGGCAGCGGC
CAATAGCAGCTTTGCTCCTTCGCTTTCTGGGCTCAGAGGCTGGGAAGGGGTGGGTCCGGGGGCGGGCTCA
GGGGCGGGCTCAGGGGCGGGGCGGGCGCCCGAAGGTCCTCCGGAGGCCCGGCATTCTGCACGCTTCAAAA
ACGCACGTCTGCCGCGCTGTTCTCCTCTTCCTCATCTCCGGGCCTTTCGACCT
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文献和实验1.常用载体分类：2,常用载体元件介绍：(1) 启动子：启动子是位于结构基因5'端上游的DNA序列，能活化RNA聚合酶，使之与模板DNA准确的结合并具有转录起始的特异性。启动子主要分为广谱启动子和特异性启动子。广谱启动子为在各种组织、细胞上广泛表达的启动子；特异性启动子为在特异性组织或者细胞类型上表达的启动子。常用广谱启动子有CAG，CMV，EF1a，PGK等，还包含启动RNA的III型启动子U6和H1启动子；常用特异性启动子如成熟神经元特异性启动子hSyn，投射神经元特异性启动
Construction of Gene-Targeting Vectors by Recombineering
by two rare cutter sites, I-SceI and I-CeuI, is targeted upstream of the intended deleted region. Subsequently, the lox P-F3-PGK-EM7-Neo-F3 (Neo ) cassette is targeted downstream of the deletion region (matching colored bands represent homology arms used
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
(not to exceed 10 μl) per 0.5 ml tube for each transfection. Origene MicroRNA Expression Plasmids (Neomycin – G418 selection) or self-cloned miRNA genes in pBABE-Puro (Plasmid 1764; Addgene) have been used successfully. For the latter, the miRNA hairpin
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