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- 江蓝纯
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- 2025年07月05日
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99
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江蓝纯
- 规格：
50ul/100ul
| 规格： | 50ul | 产品价格： | ¥960.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ¥1600.0 |
| 英文名称 | Rabbit Symmetric DiMethyl-Histone H3-R8 Polyclonal Antibody |
|---|---|
| 别名 | H3.4;H3/g;H3FT;H3t |
| 应用 | WB,IHC,IF,IP,ChIP,ChIPseq |
| 稀释比例 | WB1:500-1:2000 IHC1:50-1:200 IF1:50-1:200 IP1:50-1:200CH IP1:20-1:50CHIPseq 1:20-1:50 |
| 交叉反应 | Human,Mouse,Rat,Other,(Wide,Range) |
| 蛋白分子量 | 16kDa |
| Gene ID | 8290 |
| 保存 | Store at -20°C. Avoid freeze / thaw cycles.Stable for one year from the date of shipment. |
| 储存液 | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| 纯化方法 | Affinity purification |
| 亚型 | IgG |
| 免疫原 | A synthetic methylated peptide corresponding to residues surrounding R8 of human histone H3 |
| 性状 | Lyophilized or Liquid |
| Swiss Prot | Q16695 |
| 克隆类型 | Polyclonal Antibody |
| 来源 | Rabbit |
| 背景资料 | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. |
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文献和实验Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining
. The protocol described here allows the detection of phosphorylated histones in tissue-cultured cells and tissue sections by fluorescent or bright-field immunostaining analysis. Here we used a serine 10 specific P-histone H3 antibody to determine
FACS-Based Detection of Phosphorylated Histone H3 for the Quantitation of Mitotic Cells
scanner (FACS) is described, based on the presence of an intranuclear antigen present only in mitotic cells, detected using a specific, commercially available antibody. Cell staining and FACS analysis can be done in a single day, making this a rapid
in 100µl of elution buffer at 65°C for 5 minutes;7.The eluted complexes can then be electrophoresed in denaturing PAGE and subjected to western blot analysis with an anti-Flag antibody for detection of the particular flag-tagged protein of choice (Figure
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