pET104.1 DEST（pET104.1DEST）

pET104.1 DEST（pET104.1DEST）

质粒名称: pET104.1 DEST
其他名称:
质粒大小（bp）: 7650
抗性: Amp
质粒类型: Bacterial Expression
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平: Tightly controlled (use w/ BL21-AI)
蛋白标签: biotin (Nterm)
测序引物:
GenBank号:
其他属性: Easy to clone into other vectors
质粒图谱:

Feature Name	Start	End
pBRrevBam_primer	106	125
T7_promoter	209	227
lacO	227	254
T7_transl_en_RBS	272	288
Xpress_fwd_primer	296	314
T7_leader	297	329
Xpress_EK	564	587
attR1	597	721
attR2	721	621
lac_promoter	746	769
CAT/CamR	830	1489
ccdB	1810	2115
attR2	2156	2256
attR1	2263	2156
T7_terminator	2306	2434
T7_Terminal_primer	2366	2348
AmpR_promoter	2768	2796
Ampicillin	2838	3698
pBR322_origin	3853	4472
pGEX_3_primer	4871	4893
ROP	5078	4887
tet (611 – 0)	6328	5717
lacI	7481	6390
tet (887 – 636)	174	7573

ORF	Start	End
ORF frame 2	830	1489
ORF frame 3	2838	3698
ORF frame 3	6334	5717
ORF frame 2	5912	6607
ORF frame 2	7349	6390
ORF frame 1	72	7222

Enzyme Name	Cut
BglII	189
XbaI	255
NdeI	295
NotI	723
EcoRI	1043
NcoI	1344
AgeI	1543
SmaI	1893
XmaI	1891
SalI	2150
PacI	2290
ClaI	2599
AatII	2706
NruI	6018
HpaI	6615
ApaI	6914
BclI	7103

 

质粒序列:

1	CAAGGAGATG	GCGCCCAACA	GTCCCCCGGC	CACGGGGCCT	GCCACCATAC	50
51	CCACGCCGAA	ACAAGCGCTC	ATGAGCCCGA	AGTGGCGAGC	CCGATCTTCC	100
101	CCATCGGTGA	TGTCGGCGAT	ATAGGCGCCA	GCAACCGCAC	CTGTGGCGCC	150
151	GGTGATGCCG	GCCACGATGC	GTCCGGCGTA	GAGGATCGAG	ATCTCGATCC	200
201	CGCGAAATTA	ATACGACTCA	CTATAGGGGA	ATTGTGAGCG	GATAACAATT	250
251	CCCCTCTAGA	AATAATTTTG	TTTAACTTTA	AGAAGGAGAT	ATACATATGG	300
301	CTAGCATGAC	TGGTGGACAG	CAAATGGGTA	TTATGGGCGC	CGGCACCCCG	350
351	GTGACCGCCC	CGCTGGCGGG	CACTATCTGG	AAGGTGCTGG	CCAGCGAAGG	400
401	CCAGACGGTG	GCCGCAGGCG	AGGTGCTGCT	GATTCTGGAA	GCCATGAAGA	450
451	TGGAAACCGA	AATCCGCGCC	GCGCAGGCCG	GGACCGTGCG	CGGTATCGCG	500
501	GTGAAAGCCG	GCGACGCGGT	GGCGGTCGGC	GACACCCTGA	TGACCCTGGC	550
551	GGGCTCTGGA	TCCGATCTGT	ACGACGATGA	CGATAAGGGA	ATTATCACAA	600
601	GTTTGTACAA	AAAAGCTGAA	CGAGAAACGT	AAAATGATAT	AAATATCAAT	650
651	ATATTAAATT	AGATTTTGCA	TAAAAAACAG	ACTACATAAT	ACTGTAAAAC	700
701	ACAACATATC	CAGTCACTAT	GGCGGCCGCA	TTAGGCACCC	CAGGCTTTAC	750
751	ACTTTATGCT	TCCGGCTCGT	ATAATGTGTG	GATTTTGAGT	TAGGATCCGT	800
801	CGAGATTTTC	AGGAGCTAAG	GAAGCTAAAA	TGGAGAAAAA	AATCACTGGA	850
851	TATACCACCG	TTGATATATC	CCAATGGCAT	CGTAAAGAAC	ATTTTGAGGC	900
901	ATTTCAGTCA	GTTGCTCAAT	GTACCTATAA	CCAGACCGTT	CAGCTGGATA	950
951	TTACGGCCTT	TTTAAAGACC	GTAAAGAAAA	ATAAGCACAA	GTTTTATCCG	1000
1001	GCCTTTATTC	ACATTCTTGC	CCGCCTGATG	AATGCTCATC	CGGAATTCCG	1050
1051	TATGGCAATG	AAAGACGGTG	AGCTGGTGAT	ATGGGATAGT	GTTCACCCTT	1100
1101	GTTACACCGT	TTTCCATGAG	CAAACTGAAA	CGTTTTCATC	GCTCTGGAGT	1150
1151	GAATACCACG	ACGATTTCCG	GCAGTTTCTA	CACATATATT	CGCAAGATGT	1200
1201	GGCGTGTTAC	GGTGAAAACC	TGGCCTATTT	CCCTAAAGGG	TTTATTGAGA	1250
1251	ATATGTTTTT	CGTCTCAGCC	AATCCCTGGG	TGAGTTTCAC	CAGTTTTGAT	1300
1301	TTAAACGTGG	CCAATATGGA	CAACTTCTTC	GCCCCCGTTT	TCACCATGGG	1350
1351	CAAATATTAT	ACGCAAGGCG	ACAAGGTGCT	GATGCCGCTG	GCGATTCAGG	1400
1401	TTCATCATGC	CGTCTGTGAT	GGCTTCCATG	TCGGCAGAAT	GCTTAATGAA	1450
1451	TTACAACAGT	ACTGCGATGA	GTGGCAGGGC	GGGGCGTAAA	CGCGTGGATC	1500
1501	CGGCTTACTA	AAAGCCAGAT	AACAGTATGC	GTATTTGCGC	GCACCGGTGC	1550
1551	TAGCGTATAC	CCGAAGTATG	TCAAAAAGAG	GTGTGCTATG	AAGCAGCGTA	1600
1601	TTACAGTGAC	AGTTGACAGC	GACAGCTATC	AGTTGCTCAA	GGCATATATG	1650
1651	ATGTCAATAT	CTCCGGTCTG	GTAAGCACAA	CCATGCAGAA	TGAAGCCCGT	1700
1701	CGTCTGCGTG	CCGAACGCTG	GAAAGCGGAA	AATCAGGAAG	GGATGGCTGA	1750
1751	GGTCGCCCGG	TTTATTGAAA	TGAACGGCTC	TTTTGCTGAC	GAGAACAGGG	1800
1801	ACTGGTGAAA	TGCAGTTTAA	GGTTTACACC	TATAAAAGAG	AGAGCCGTTA	1850
1851	TCGTCTGTTT	GTGGATGTAC	AGAGTGATAT	TATTGACACG	CCCGGGCGAC	1900
1901	GGATGGTGAT	CCCCCTGGCC	AGTGCACGTC	TGCTGTCAGA	TAAAGTCTCC	1950
1951	CGTGAACTTT	ACCCGGTGGT	GCATATCGGG	GATGAAAGCT	GGCGCATGAT	2000
2001	GACCACCGAT	ATGGCCAGTG	TGCCGGTCTC	CGTTATCGGG	GAAGAAGTGG	2050
2051	CTGATCTCAG	CCACCGCGAA	AATGACATCA	AAAACGCCAT	TAACCTGATG	2100
2101	TTCTGGGGAA	TATAAATGTC	AGGCTCCGTT	ATACACAGCC	AGTCTGCAGG	2150
2151	TCGACCATAG	TGACTGGATA	TGTTGTGTTT	TACAGTATTA	TGTAGTCTGT	2200
2201	TTTTTATGCA	AAATCTAATT	TAATATATTG	ATATTTATAT	CATTTTACGT	2250
2251	TTCTCGTTCA	GCTTTCTTGT	ACAAAGTGGT	GATAATTAAT	TAAGATCAGA	2300
2301	TCCGGCTGCT	AACAAAGCCC	GAAAGGAAGC	TGAGTTGGCT	GCTGCCACCG	2350
2351	CTGAGCAATA	ACTAGCATAA	CCCCTTGGGG	CCTCTAAACG	GGTCTTGAGG	2400
2401	GGTTTTTTGC	TGAAAGGAGG	AACTATATCC	GGATATCCCG	CAAGAGGCCC	2450
2451	GGCAGTACCG	GCATAACCAA	GCCTATGCCT	ACAGCATCCA	GGGTGACGGT	2500
2501	GCCGAGGATG	ACGATGAGCG	CATTGTTAGA	TTTCATACAC	GGTGCCTGAC	2550
2551	TGCGTTAGCA	ATTTAACTGT	GATAAACTAC	CGCATTAAAG	CTAGCTTATC	2600
2601	GATGATAAGC	TGTCAAACAT	GAGAATTAAT	TCTTGAAGAC	GAAAGGGCCT	2650
2651	CGTGATACGC	CTATTTTTAT	AGGTTAATGT	CATGATAATA	ATGGTTTCTT	2700
2701	AGACGTCAGG	TGGCACTTTT	CGGGGAAATG	TGCGCGGAAC	CCCTATTTGT	2750
2751	TTATTTTTCT	AAATACATTC	AAATATGTAT	CCGCTCATGA	GACAATAACC	2800
2801	CTGATAAATG	CTTCAATAAT	ATTGAAAAAG	GAAGAGTATG	AGTATTCAAC	2850
2851	ATTTCCGTGT	CGCCCTTATT	CCCTTTTTTG	CGGCATTTTG	CCTTCCTGTT	2900
2901	TTTGCTCACC	CAGAAACGCT	GGTGAAAGTA	AAAGATGCTG	AAGATCAGTT	2950
2951	GGGTGCACGA	GTGGGTTACA	TCGAACTGGA	TCTCAACAGC	GGTAAGATCC	3000
3001	TTGAGAGTTT	TCGCCCCGAA	GAACGTTTTC	CAATGATGAG	CACTTTTAAA	3050
3051	GTTCTGCTAT	GTGGCGCGGT	ATTATCCCGT	GTTGACGCCG	GGCAAGAGCA	3100
3101	ACTCGGTCGC	CGCATACACT	ATTCTCAGAA	TGACTTGGTT	GAGTACTCAC	3150
3151	CAGTCACAGA	AAAGCATCTT	ACGGATGGCA	TGACAGTAAG	AGAATTATGC	3200
3201	AGTGCTGCCA	TAACCATGAG	TGATAACACT	GCGGCCAACT	TACTTCTGAC	3250
3251	AACGATCGGA	GGACCGAAGG	AGCTAACCGC	TTTTTTGCAC	AACATGGGGG	3300
3301	ATCATGTAAC	TCGCCTTGAT	CGTTGGGAAC	CGGAGCTGAA	TGAAGCCATA	3350
3351	CCAAACGACG	AGCGTGACAC	CACGATGCCT	GCAGCAATGG	CAACAACGTT	3400
3401	GCGCAAACTA	TTAACTGGCG	AACTACTTAC	TCTAGCTTCC	CGGCAACAAT	3450
3451	TAATAGACTG	GATGGAGGCG	GATAAAGTTG	CAGGACCACT	TCTGCGCTCG	3500
3501	GCCCTTCCGG	CTGGCTGGTT	TATTGCTGAT	AAATCTGGAG	CCGGTGAGCG	3550
3551	TGGGTCTCGC	GGTATCATTG	CAGCACTGGG	GCCAGATGGT	AAGCCCTCCC	3600
3601	GTATCGTAGT	TATCTACACG	ACGGGGAGTC	AGGCAACTAT	GGATGAACGA	3650
3651	AATAGACAGA	TCGCTGAGAT	AGGTGCCTCA	CTGATTAAGC	ATTGGTAACT	3700
3701	GTCAGACCAA	GTTTACTCAT	ATATACTTTA	GATTGATTTA	AAACTTCATT	3750
3751	TTTAATTTAA	AAGGATCTAG	GTGAAGATCC	TTTTTGATAA	TCTCATGACC	3800
3801	AAAATCCCTT	AACGTGAGTT	TTCGTTCCAC	TGAGCGTCAG	ACCCCGTAGA	3850
3851	AAAGATCAAA	GGATCTTCTT	GAGATCCTTT	TTTTCTGCGC	GTAATCTGCT	3900
3901	GCTTGCAAAC	AAAAAAACCA	CCGCTACCAG	CGGTGGTTTG	TTTGCCGGAT	3950
3951	CAAGAGCTAC	CAACTCTTTT	TCCGAAGGTA	ACTGGCTTCA	GCAGAGCGCA	4000
4001	GATACCAAAT	ACTGTCCTTC	TAGTGTAGCC	GTAGTTAGGC	CACCACTTCA	4050
4051	AGAACTCTGT	AGCACCGCCT	ACATACCTCG	CTCTGCTAAT	CCTGTTACCA	4100
4101	GTGGCTGCTG	CCAGTGGCGA	TAAGTCGTGT	CTTACCGGGT	TGGACTCAAG	4150
4151	ACGATAGTTA	CCGGATAAGG	CGCAGCGGTC	GGGCTGAACG	GGGGGTTCGT	4200
4201	GCACACAGCC	CAGCTTGGAG	CGAACGACCT	ACACCGAACT	GAGATACCTA	4250
4251	CAGCGTGAGC	TATGAGAAAG	CGCCACGCTT	CCCGAAGGGA	GAAAGGCGGA	4300
4301	CAGGTATCCG	GTAAGCGGCA	GGGTCGGAAC	AGGAGAGCGC	ACGAGGGAGC	4350
4351	TTCCAGGGGG	AAACGCCTGG	TATCTTTATA	GTCCTGTCGG	GTTTCGCCAC	4400
4401	CTCTGACTTG	AGCGTCGATT	TTTGTGATGC	TCGTCAGGGG	GGCGGAGCCT	4450
4451	ATGGAAAAAC	GCCAGCAACG	CGGCCTTTTT	ACGGTTCCTG	GCCTTTTGCT	4500
4501	GGCCTTTTGC	TCACATGTTC	TTTCCTGCGT	TATCCCCTGA	TTCTGTGGAT	4550
4551	AACCGTATTA	CCGCCTTTGA	GTGAGCTGAT	ACCGCTCGCC	GCAGCCGAAC	4600
4601	GACCGAGCGC	AGCGAGTCAG	TGAGCGAGGA	AGCGGAAGAG	CGCCTGATGC	4650
4651	GGTATTTTCT	CCTTACGCAT	CTGTGCGGTA	TTTCACACCG	CATATATGGT	4700
4701	GCACTCTCAG	TACAATCTGC	TCTGATGCCG	CATAGTTAAG	CCAGTATACA	4750
4751	CTCCGCTATC	GCTACGTGAC	TGGGTCATGG	CTGCGCCCCG	ACACCCGCCA	4800
4801	ACACCCGCTG	ACGCGCCCTG	ACGGGCTTGT	CTGCTCCCGG	CATCCGCTTA	4850
4851	CAGACAAGCT	GTGACCGTCT	CCGGGAGCTG	CATGTGTCAG	AGGTTTTCAC	4900
4901	CGTCATCACC	GAAACGCGCG	AGGCAGCTGC	GGTAAAGCTC	ATCAGCGTGG	4950
4951	TCGTGAAGCG	ATTCACAGAT	GTCTGCCTGT	TCATCCGCGT	CCAGCTCGTT	5000
5001	GAGTTTCTCC	AGAAGCGTTA	ATGTCTGGCT	TCTGATAAAG	CGGGCCATGT	5050
5051	TAAGGGCGGT	TTTTTCCTGT	TTGGTCACTG	ATGCCTCCGT	GTAAGGGGGA	5100
5101	TTTCTGTTCA	TGGGGGTAAT	GATACCGATG	AAACGAGAGA	GGATGCTCAC	5150
5151	GATACGGGTT	ACTGATGATG	AACATGCCCG	GTTACTGGAA	CGTTGTGAGG	5200
5201	GTAAACAACT	GGCGGTATGG	ATGCGGCGGG	ACCAGAGAAA	AATCACTCAG	5250
5251	GGTCAATGCC	AGCGCTTCGT	TAATACAGAT	GTAGGTGTTC	CACAGGGTAG	5300
5301	CCAGCAGCAT	CCTGCGATGC	AGATCCGGAA	CATAATGGTG	CAGGGCGCTG	5350
5351	ACTTCCGCGT	TTCCAGACTT	TACGAAACAC	GGAAACCGAA	GACCATTCAT	5400
5401	GTTGTTGCTC	AGGTCGCAGA	CGTTTTGCAG	CAGCAGTCGC	TTCACGTTCG	5450
5451	CTCGCGTATC	GGTGATTCAT	TCTGCTAACC	AGTAAGGCAA	CCCCGCCAGC	5500
5501	CTAGCCGGGT	CCTCAACGAC	AGGAGCACGA	TCATGCGCAC	CCGTGGCCAG	5550
5551	GACCCAACGC	TGCCCGAGAT	GCGCCGCGTG	CGGCTGCTGG	AGATGGCGGA	5600
5601	CGCGATGGAT	ATGTTCTGCC	AAGGGTTGGT	TTGCGCATTC	ACAGTTCTCC	5650
5651	GCAAGAATTG	ATTGGCTCCA	ATTCTTGGAG	TGGTGAATCC	GTTAGCGAGG	5700
5701	TGCCGCCGGC	TTCCATTCAG	GTCGAGGTGG	CCCGGCTCCA	TGCACCGCGA	5750
5751	CGCAACGCGG	GGAGGCAGAC	AAGGTATAGG	GCGGCGCCTA	CAATCCATGC	5800
5801	CAACCCGTTC	CATGTGCTCG	CCGAGGCGGC	ATAAATCGCC	GTGACGATCA	5850
5851	GCGGTCCAGT	GATCGAAGTT	AGGCTGGTAA	GAGCCGCGAG	CGATCCTTGA	5900
5901	AGCTGTCCCT	GATGGTCGTC	ATCTACCTGC	CTGGACAGCA	TGGCCTGCAA	5950
5951	CGCGGGCATC	CCGATGCCGC	CGGAAGCGAG	AAGAATCATA	ATGGGGAAGG	6000
6001	CCATCCAGCC	TCGCGTCGCG	AACGCCAGCA	AGACGTAGCC	CAGCGCGTCG	6050
6051	GCCGCCATGC	CGGCGATAAT	GGCCTGCTTC	TCGCCGAAAC	GTTTGGTGGC	6100
6101	GGGACCAGTG	ACGAAGGCTT	GAGCGAGGGC	GTGCAAGATT	CCGAATACCG	6150
6151	CAAGCGACAG	GCCGATCATC	GTCGCGCTCC	AGCGAAAGCG	GTCCTCGCCG	6200
6201	AAAATGACCC	AGAGCGCTGC	CGGCACCTGT	CCTACGAGTT	GCATGATAAA	6250
6251	GAAGACAGTC	ATAAGTGCGG	CGACGATAGT	CATGCCCCGC	GCCCACCGGA	6300
6301	AGGAGCTGAC	TGGGTTGAAG	GCTCTCAAGG	GCATCGGTCG	AGATCCCGGT	6350
6351	GCCTAATGAG	TGAGCTAACT	TACATTAATT	GCGTTGCGCT	CACTGCCCGC	6400
6401	TTTCCAGTCG	GGAAACCTGT	CGTGCCAGCT	GCATTAATGA	ATCGGCCAAC	6450
6451	GCGCGGGGAG	AGGCGGTTTG	CGTATTGGGC	GCCAGGGTGG	TTTTTCTTTT	6500
6501	CACCAGTGAG	ACGGGCAACA	GCTGATTGCC	CTTCACCGCC	TGGCCCTGAG	6550
6551	AGAGTTGCAG	CAAGCGGTCC	ACGCTGGTTT	GCCCCAGCAG	GCGAAAATCC	6600
6601	TGTTTGATGG	TGGTTAACGG	CGGGATATAA	CATGAGCTGT	CTTCGGTATC	6650
6651	GTCGTATCCC	ACTACCGAGA	TATCCGCACC	AACGCGCAGC	CCGGACTCGG	6700
6701	TAATGGCGCG	CATTGCGCCC	AGCGCCATCT	GATCGTTGGC	AACCAGCATC	6750
6751	GCAGTGGGAA	CGATGCCCTC	ATTCAGCATT	TGCATGGTTT	GTTGAAAACC	6800
6801	GGACATGGCA	CTCCAGTCGC	CTTCCCGTTC	CGCTATCGGC	TGAATTTGAT	6850
6851	TGCGAGTGAG	ATATTTATGC	CAGCCAGCCA	GACGCAGACG	CGCCGAGACA	6900
6901	GAACTTAATG	GGCCCGCTAA	CAGCGCGATT	TGCTGGTGAC	CCAATGCGAC	6950
6951	CAGATGCTCC	ACGCCCAGTC	GCGTACCGTC	TTCATGGGAG	AAAATAATAC	7000
7001	TGTTGATGGG	TGTCTGGTCA	GAGACATCAA	GAAATAACGC	CGGAACATTA	7050
7051	GTGCAGGCAG	CTTCCACAGC	AATGGCATCC	TGGTCATCCA	GCGGATAGTT	7100
7101	AATGATCAGC	CCACTGACGC	GTTGCGCGAG	AAGATTGTGC	ACCGCCGCTT	7150
7151	TACAGGCTTC	GACGCCGCTT	CGTTCTACCA	TCGACACCAC	CACGCTGGCA	7200
7201	CCCAGTTGAT	CGGCGCGAGA	TTTAATCGCC	GCGACAATTT	GCGACGGCGC	7250
7251	GTGCAGGGCC	AGACTGGAGG	TGGCAACGCC	AATCAGCAAC	GACTGTTTGC	7300
7301	CCGCCAGTTG	TTGTGCCACG	CGGTTGGGAA	TGTAATTCAG	CTCCGCCATC	7350
7351	GCCGCTTCCA	CTTTTTCCCG	CGTTTTCGCA	GAAACGTGGC	TGGCCTGGTT	7400
7401	CACCACGCGG	GAAACGGTCT	GATAAGAGAC	ACCGGCATAC	TCTGCGACAT	7450
7451	CGTATAACGT	TACTGGTTTC	ACATTCACCA	CCCTGAATTG	ACTCTCTTCC	7500
7501	GGGCGCTATC	ATGCCATACC	GCGAAAGGTT	TTGCGCCATT	CGATGGTGTC	7550
7551	CGGGATCTCG	ACGCTCTCCC	TTATGCGACT	CCTGCATTAG	GAAGCAGCCC	7600
7601	AGTAGTAGGT	TGAGGCCGTT	GAGCACCGCC	GCCGCAAGGA	ATGGTGCATG	7650