pEGFP-C1

pEGFP-C1

质粒名称: pEGFP-C1
其他名称:
质粒大小（bp）: 4731
抗性: Kanamycin (卡那霉素)
质粒类型: 哺乳动物细胞表达载体
是否病毒: 非病毒
启动子: CMV
稳转或瞬转: 稳转
组成型或诱导性: 组成型
表达水平: 高
蛋白标签:N-EGFP
5’测序引物:pEGFP-C-5′: 5′-CATGGTCCTGCTGGAGTTCGTG-3′

3’测序引物:pEGFP-C-3′: 5′-TATGGCTGATTATGATCAGT-3′ ;
pEGFP-C1-R: 5′-CTACAAATGTGGTATGGCTG-3′
GenBank号:
其他属性:

Vector for fusing EGFP to the N-terminus of a partner protein.

质粒图谱:

质粒序列:

1	TAGTTATTAA	TAGTAATCAA	TTACGGGGTC	ATTAGTTCAT	AGCCCATATA	TGGAGTTCCG
61	CGTTACATAA	CTTACGGTAA	ATGGCCCGCC	TGGCTGACCG	CCCAACGACC	CCCGCCCATT
121	GACGTCAATA	ATGACGTATG	TTCCCATAGT	AACGCCAATA	GGGACTTTCC	ATTGACGTCA
181	ATGGGTGGAG	TATTTACGGT	AAACTGCCCA	CTTGGCAGTA	CATCAAGTGT	ATCATATGCC
241	AAGTACGCCC	CCTATTGACG	TCAATGACGG	TAAATGGCCC	GCCTGGCATT	ATGCCCAGTA
301	CATGACCTTA	TGGGACTTTC	CTACTTGGCA	GTACATCTAC	GTATTAGTCA	TCGCTATTAC
361	CATGGTGATG	CGGTTTTGGC	AGTACATCAA	TGGGCGTGGA	TAGCGGTTTG	ACTCACGGGG
421	ATTTCCAAGT	CTCCACCCCA	TTGACGTCAA	TGGGAGTTTG	TTTTGGCACC	AAAATCAACG
481	GGACTTTCCA	AAATGTCGTA	ACAACTCCGC	CCCATTGACG	CAAATGGGCG	GTAGGCGTGT
541	ACGGTGGGAG	GTCTATATAA	GCAGAGCTGG	TTTAGTGAAC	CGTCAGATCC	GCTAGCGCTA
601	CCGGTCGCCA	CCATGGTGAG	CAAGGGCGAG	GAGCTGTTCA	CCGGGGTGGT	GCCCATCCTG
661	GTCGAGCTGG	ACGGCGACGT	AAACGGCCAC	AAGTTCAGCG	TGTCCGGCGA	GGGCGAGGGC
721	GATGCCACCT	ACGGCAAGCT	GACCCTGAAG	TTCATCTGCA	CCACCGGCAA	GCTGCCCGTG
781	CCCTGGCCCA	CCCTCGTGAC	CACCCTGACC	TACGGCGTGC	AGTGCTTCAG	CCGCTACCCC
841	GACCACATGA	AGCAGCACGA	CTTCTTCAAG	TCCGCCATGC	CCGAAGGCTA	CGTCCAGGAG
901	CGCACCATCT	TCTTCAAGGA	CGACGGCAAC	TACAAGACCC	GCGCCGAGGT	GAAGTTCGAG
961	GGCGACACCC	TGGTGAACCG	CATCGAGCTG	AAGGGCATCG	ACTTCAAGGA	GGACGGCAAC
1021	ATCCTGGGGC	ACAAGCTGGA	GTACAACTAC	AACAGCCACA	ACGTCTATAT	CATGGCCGAC
1081	AAGCAGAAGA	ACGGCATCAA	GGTGAACTTC	AAGATCCGCC	ACAACATCGA	GGACGGCAGC
1141	GTGCAGCTCG	CCGACCACTA	CCAGCAGAAC	ACCCCCATCG	GCGACGGCCC	CGTGCTGCTG
1201	CCCGACAACC	ACTACCTGAG	CACCCAGTCC	GCCCTGAGCA	AAGACCCCAA	CGAGAAGCGC
1261	GATCACATGG	TCCTGCTGGA	GTTCGTGACC	GCCGCCGGGA	TCACTCTCGG	CATGGACGAG
1321	CTGTACAAGT	CCGGACTCAG	ATCTCGAGCT	CAAGCTTCGA	ATTCTGCAGT	CGACGGTACC
1381	GCGGGCCCGG	GATCCACCGG	ATCTAGATAA	CTGATCATAA	TCAGCCATAC	CACATTTGTA
1441	GAGGTTTTAC	TTGCTTTAAA	AAACCTCCCA	CACCTCCCCC	TGAACCTGAA	ACATAAAATG
1501	AATGCAATTG	TTGTTGTTAA	CTTGTTTATT	GCAGCTTATA	ATGGTTACAA	ATAAAGCAAT
1561	AGCATCACAA	ATTTCACAAA	TAAAGCATTT	TTTTCACTGC	ATTCTAGTTG	TGGTTTGTCC
1621	AAACTCATCA	ATGTATCTTA	ACGCGTAAAT	TGTAAGCGTT	AATATTTTGT	TAAAATTCGC
1681	GTTAAATTTT	TGTTAAATCA	GCTCATTTTT	TAACCAATAG	GCCGAAATCG	GCAAAATCCC
1741	TTATAAATCA	AAAGAATAGA	CCGAGATAGG	GTTGAGTGTT	GTTCCAGTTT	GGAACAAGAG
1801	TCCACTATTA	AAGAACGTGG	ACTCCAACGT	CAAAGGGCGA	AAAACCGTCT	ATCAGGGCGA
1861	TGGCCCACTA	CGTGAACCAT	CACCCTAATC	AAGTTTTTTG	GGGTCGAGGT	GCCGTAAAGC
1921	ACTAAATCGG	AACCCTAAAG	GGAGCCCCCG	ATTTAGAGCT	TGACGGGGAA	AGCCGGCGAA
1981	CGTGGCGAGA	AAGGAAGGGA	AGAAAGCGAA	AGGAGCGGGC	GCTAGGGCGC	TGGCAAGTGT
2041	AGCGGTCACG	CTGCGCGTAA	CCACCACACC	CGCCGCGCTT	AATGCGCCGC	TACAGGGCGC
2101	GTCAGGTGGC	ACTTTTCGGG	GAAATGTGCG	CGGAACCCCT	ATTTGTTTAT	TTTTCTAAAT
2161	ACATTCAAAT	ATGTATCCGC	TCATGAGACA	ATAACCCTGA	TAAATGCTTC	AATAATATTG
2221	AAAAAGGAAG	AGTCCTGAGG	CGGAAAGAAC	CAGCTGTGGA	ATGTGTGTCA	GTTAGGGTGT
2281	GGAAAGTCCC	CAGGCTCCCC	AGCAGGCAGA	AGTATGCAAA	GCATGCATCT	CAATTAGTCA
2341	GCAACCAGGT	GTGGAAAGTC	CCCAGGCTCC	CCAGCAGGCA	GAAGTATGCA	AAGCATGCAT
2401	CTCAATTAGT	CAGCAACCAT	AGTCCCGCCC	CTAACTCCGC	CCATCCCGCC	CCTAACTCCG
2461	CCCAGTTCCG	CCCATTCTCC	GCCCCATGGC	TGACTAATTT	TTTTTATTTA	TGCAGAGGCC
2521	GAGGCCGCCT	CGGCCTCTGA	GCTATTCCAG	AAGTAGTGAG	GAGGCTTTTT	TGGAGGCCTA
2581	GGCTTTTGCA	AAGATCGATC	AAGAGACAGG	ATGAGGATCG	TTTCGCATGA	TTGAACAAGA
2641	TGGATTGCAC	GCAGGTTCTC	CGGCCGCTTG	GGTGGAGAGG	CTATTCGGCT	ATGACTGGGC
2701	ACAACAGACA	ATCGGCTGCT	CTGATGCCGC	CGTGTTCCGG	CTGTCAGCGC	AGGGGCGCCC
2761	GGTTCTTTTT	GTCAAGACCG	ACCTGTCCGG	TGCCCTGAAT	GAACTGCAAG	ACGAGGCAGC
2821	GCGGCTATCG	TGGCTGGCCA	CGACGGGCGT	TCCTTGCGCA	GCTGTGCTCG	ACGTTGTCAC
2881	TGAAGCGGGA	AGGGACTGGC	TGCTATTGGG	CGAAGTGCCG	GGGCAGGATC	TCCTGTCATC
2941	TCACCTTGCT	CCTGCCGAGA	AAGTATCCAT	CATGGCTGAT	GCAATGCGGC	GGCTGCATAC
3001	GCTTGATCCG	GCTACCTGCC	CATTCGACCA	CCAAGCGAAA	CATCGCATCG	AGCGAGCACG
3061	TACTCGGATG	GAAGCCGGTC	TTGTCGATCA	GGATGATCTG	GACGAAGAGC	ATCAGGGGCT
3121	CGCGCCAGCC	GAACTGTTCG	CCAGGCTCAA	GGCGAGCATG	CCCGACGGCG	AGGATCTCGT
3181	CGTGACCCAT	GGCGATGCCT	GCTTGCCGAA	TATCATGGTG	GAAAATGGCC	GCTTTTCTGG
3241	ATTCATCGAC	TGTGGCCGGC	TGGGTGTGGC	GGACCGCTAT	CAGGACATAG	CGTTGGCTAC
3301	CCGTGATATT	GCTGAAGAGC	TTGGCGGCGA	ATGGGCTGAC	CGCTTCCTCG	TGCTTTACGG
3361	TATCGCCGCT	CCCGATTCGC	AGCGCATCGC	CTTCTATCGC	CTTCTTGACG	AGTTCTTCTG
3421	AGCGGGACTC	TGGGGTTCGA	AATGACCGAC	CAAGCGACGC	CCAACCTGCC	ATCACGAGAT
3481	TTCGATTCCA	CCGCCGCCTT	CTATGAAAGG	TTGGGCTTCG	GAATCGTTTT	CCGGGACGCC
3541	GGCTGGATGA	TCCTCCAGCG	CGGGGATCTC	ATGCTGGAGT	TCTTCGCCCA	CCCTAGGGGG
3601	AGGCTAACTG	AAACACGGAA	GGAGACAATA	CCGGAAGGAA	CCCGCGCTAT	GACGGCAATA
3661	AAAAGACAGA	ATAAAACGCA	CGGTGTTGGG	TCGTTTGTTC	ATAAACGCGG	GGTTCGGTCC
3721	CAGGGCTGGC	ACTCTGTCGA	TACCCCACCG	AGACCCCATT	GGGGCCAATA	CGCCCGCGTT
3781	TCTTCCTTTT	CCCCACCCCA	CCCCCCAAGT	TCGGGTGAAG	GCCCAGGGCT	CGCAGCCAAC
3841	GTCGGGGCGG	CAGGCCCTGC	CATAGCCTCA	GGTTACTCAT	ATATACTTTA	GATTGATTTA
3901	AAACTTCATT	TTTAATTTAA	AAGGATCTAG	GTGAAGATCC	TTTTTGATAA	TCTCATGACC
3961	AAAATCCCTT	AACGTGAGTT	TTCGTTCCAC	TGAGCGTCAG	ACCCCGTAGA	AAAGATCAAA
4021	GGATCTTCTT	GAGATCCTTT	TTTTCTGCGC	GTAATCTGCT	GCTTGCAAAC	AAAAAAACCA
4081	CCGCTACCAG	CGGTGGTTTG	TTTGCCGGAT	CAAGAGCTAC	CAACTCTTTT	TCCGAAGGTA
4141	ACTGGCTTCA	GCAGAGCGCA	GATACCAAAT	ACTGTCCTTC	TAGTGTAGCC	GTAGTTAGGC
4201	CACCACTTCA	AGAACTCTGT	AGCACCGCCT	ACATACCTCG	CTCTGCTAAT	CCTGTTACCA
4261	GTGGCTGCTG	CCAGTGGCGA	TAAGTCGTGT	CTTACCGGGT	TGGACTCAAG	ACGATAGTTA
4321	CCGGATAAGG	CGCAGCGGTC	GGGCTGAACG	GGGGGTTCGT	GCACACAGCC	CAGCTTGGAG
4381	CGAACGACCT	ACACCGAACT	GAGATACCTA	CAGCGTGAGC	TATGAGAAAG	CGCCACGCTT
4441	CCCGAAGGGA	GAAAGGCGGA	CAGGTATCCG	GTAAGCGGCA	GGGTCGGAAC	AGGAGAGCGC
4501	ACGAGGGAGC	TTCCAGGGGG	AAACGCCTGG	TATCTTTATA	GTCCTGTCGG	GTTTCGCCAC
4561	CTCTGACTTG	AGCGTCGATT	TTTGTGATGC	TCGTCAGGGG	GGCGGAGCCT	ATGGAAAAAC
4621	GCCAGCAACG	CGGCCTTTTT	ACGGTTCCTG	GCCTTTTGCT	GGCCTTTTGC	TCACATGTTC
4681	TTTCCTGCGT	TATCCCCTGA	TTCTGTGGAT	AACCGTATTA	CCGCCATGCA	T
4731