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- 库存:
99
- 供应商:
江蓝纯
- 规格:
50ul/100ul
| 规格: | 50ul | 产品价格: | ¥960.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1600.0 |
| 英文名称 | Mouse Caspase-9 Monoclonal Antibody |
|---|---|
| 别名 | Caspase-9 |
| 应用 | WB |
| 稀释比例 | WB1:1000 ICC |
| 交叉反应 | Human,Transfected |
| 蛋白分子量 | 49,37kDa |
| Gene ID | 842 |
| 保存 | Store at -20°C. Avoid freeze / thaw cycles.Stable for one year from the date of shipment. |
| 储存液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 69% Glycerol. |
| 纯化方法 | Affinity purification |
| 亚型 | IgG1 |
| 免疫原 | Purified recombinant human Caspase-9 protein fragments expressed in E.coli. |
| 性状 | Lyophilized or Liquid |
| Swiss Prot | P55211 |
| 克隆号 | 1D1-F2-E9 |
| 克隆类型 | Monoclonal Antibody |
| 来源 | Mouse |
| 背景资料 | This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. |
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文献和实验相关实验
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma,在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应,对应于抗原之不同部分可制成各种不同的抗体复合体。然而,因为一个产生抗体的细胞,只能产生一种抗体,所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种,可形成边产生抗体边增殖的 hybridoma,将每个杂种细胞进行克隆(无性繁殖系)培养,则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成,这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
a simple ELISA method to detect human anti-antibody response.
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Anti-Caspase-9 Monoclonal Antibody
¥960 - 1600




