Anti-CaV1.2-ATTO-488

Anti-Ca_V1.2-ATTO-488

a_1c, L-type of Voltage-Gated Ca²⁺ Channel, Cacna1c
Cat #: ACC-1003-AG
Sizes: 50 µl
Source: Rabbit
Type: Polyclonal
Applications: IC, IH
Reactivity: H, M, R

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Ca_V1.2 channel. Anti-Ca_V1.2 antibody (#ACC-003) can be used in western blot, immunoprecipitation, immunohistochemistry, immunocytochemistry and indirect flow cytometry applications. It has been designed to recognize Ca_V1.2 from mouse, rat and human samples.

Alomone Labs is pleased to offer a new version of this antibody, Anti-Ca_v1.2-ATTO-488 antibody (#ACC-003-AG), that is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Ca_v1.2-ATTO-488 antibody has been tested in immunohistochemical and immunocytochemical applications and is specially suited for experiments requiring simultaneous labeling of different markers.

• Applications
• Specifications
• Scientific Background
• Related Products

Immunohistochemistry

Expression of Ca_v1.2 in rat cerebellum
Immunohistochemical staining of rat cerebellum frozen sections with Anti-Ca_v1.2-ATTO-488 antibody (#ACC-003-AG), (1:100). Both dendrites of Purkinje cells (horizontal arrows) and fibers of Bergmann glia (vertical arrows) were stained. DAPI (blue Nissl counterstain) helps define the layers: granule (G), Purkinje (P) and molecular (M).

Expression of Ca_v1.2 in rat pancreas
Immunohistochemical staining of paraffin embedded sections of rat pancreas using Anti-Ca_v1.2-ATTO-488 antibody (#ACC-003-AG), (1:50). Staining is highly specific for endocrine cells of the Isle of Langerhans (IL).
Immunocytochemistry

Expression of Ca_v1.2 in mouse pancreatic microvascular endothelial cells (MS1)
Immunocytochemical staining of MS1 cells. A. Intracellular staining of paraformaldehyde-fixed and permeabilized MS1 cells with Anti-Cav1.2-ATTO-488 antibody (#ACC-003-AG), (1:50). B. The cell-permeable dye Hoechst 33342 (blue) was used for nuclear staining. C. Merged image of panels A and B.
Immunogen
Peptide (C)TTKINMDDLQPSENEDKS, corresponding to amino acid residues 848-865 of rat Ca_v1.2 (Accession P22002).  Intracellular loop between domains II and III.

HomologyMouse - identical; guinea pig - 17/18 amino acid residues identical; human, rabbit - 16/18 amino acid residues identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.
LabelATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.
Standard quality control of each lotWestern blot analysis (unlabeled antibody, #ACC-003), and immunohistochemistry (labeled antibody).
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution50 µl double distilled water (DDW).
Antibody concentration after reconstitution1 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 μg peptide per 1 μg antibody.
Scientific backgroundAll L-type calcium channels are encoded by one of the Ca_V1 channel genes. These channels play a major role as a Ca²⁺ entry pathway in skeletal cardiac and smooth muscles as well as in neurons, endocrine cells and possibly in non-excitable cells such as hematopoetic and epithelial cells. All Ca_V1 channels are influenced by dihydropyridines (DHP) and are also referred to as DHP receptor. While the Ca_V1.1 and Ca_V1.4 isoforms are expressed in restricted tissues (skeletal muscle and retina, respectively), the expression of Ca_V1.2 is ubiquitous and Ca_V1.3 channels are found in heart, brain and pancreas. Several peptidyl toxins are described that are specific L-type channels blockers, but so far no selective blocker for one of the Ca_V1 isoforms have been described. These include the Mamba toxins Calcicludine (#C-650), Calciseptine (#C-500) and FS-2 (#F-700).
References

1. Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 579.
2. IUPHAR
3. Hu, X.Q. et al. (1998) J. Biol. Chem. 273, 5337.
4. Kreuzberg, U. et al. (2000) Am J. Physiol. 278, H723.
5. Allard, B. et al. (2000) J. Biol. Chem. 275. 25556.