γ2 Subunit of Voltage-Gated Ca2+ Channel, Stargazin, Cacng2, TARP Gamma-2
Cat #: ACC-012
Sizes: 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: IC, WB
May also work in: IFC, IH, IP
Reactivity: H, M, R
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
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Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat CaVγ2. Anti-CaVγ2 antibody (#ACC-012) can be used in western blot analysis. It has been designed to recognize CaVγ2 from rat, mouse and human samples.
For a list of product citations in the literature, see product citations tab below. If you know of additional relevant citations for this product, please let us know.
Applications
Specifications
Scientific Background
Citations
Related Products
Western blot
Western blot analysis of rat brain membranes: 1. Anti-Cavγ2 antibody (#ACC-012), (1:200).
2. Anti-Cavγ2 antibody, preincubated with the control peptide antigen.
Immunocytochemistry
Rat spinal cord culture (1:200) (see Krishnamurthy, K. et al. (2013) Brain Res.1529, 143.).
Immunogen
Peptide (C)RATDYLQASAITRIPS, corresponding to amino acid residues 213-228 of mouse Cavγ2 (Accession O88602). Intracellular, C-terminus.
HomologyRat, human - identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.6 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background
The γ subunit protein is an auxiliary subunit of the CaV (voltage dependent calcium) channel multimer complex. γ1 was first identified in skeletal muscle as a part of the proteins complex purified as the DHP receptor. Eight γ subunit isoforms were identified in mammalian genoms and they share some sequence as well as structure homology.1 γ subunit contains four transmembrane helixes, with intracellular N and C termini. Usually their association with the pore-forming α1 subunit results in reduced current amplitude.
CaVγ2 is also known as stargazin, and interacts and regulates the activity and trafficking of AMPA glutamate receptors.2
References
Arikkath, J. et al. (2003) Curr. Opin. Neurobiol. 13, 298.