Anti-IP3 Receptor-2-ATTO-594

Anti-IP₃ Receptor-2-ATTO-594

IP₃R2, IP₃ receptor isoform 2, InsP3R2, Type 2, inositol 1,4,5-trisphosphate receptor
Cat #: ACC-116-AR
Sizes: 50 µl
Source: Rabbit
Type: Polyclonal
Applications: IH
May also work in: IC
Reactivity: R

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat IP₃R2. Anti-IP₃ Receptor-2 antibody (#ACC-116) can be used in western blot and immunohistochemistry applications. It has been designed to recognize IP₃R2 from rat, mouse and human samples.

We are pleased to offer a new version of this antibody that is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-IP₃ Receptor-2-ATTO-594 antibody (#ACC-116-AR) has been tested in immunohistochemical applications and is specially suited for experiments requiring simultaneous labeling of different markers.

• Applications
• Specifications
• Scientific Background
• Related Products

Immunohistochemistry

Expression of IP₃ Receptor-2 in rat spinal cord
Immunohistochemical staining of rat spinal cord using Anti-IP₃ Receptor-2-ATTO-594 antibody (#ACC-116-AR), (1:60). A. IP₃ Receptor-2 immunoreactivity (red) appears in neuronal soma (horizontal arrows) and processes (vertical arrows). B. Nuclear staining using DAPI as the counterstain (blue). C. Merged images of A and B.
Immunogen
Peptide (C)RLGFLGSNTPHENH, corresponding to amino acid residues 2683-2696 of rat IP3R2 (Accession P29995). Intracellular, C-terminus.

HomologyMouse, human - 13/14 amino acid residues identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.
Label

ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.

Standard quality control of each lotWestern blot analysis (unlabeled antibody, #ACC-116), and immunohistochemistry (labeled antibody).
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution50 µl double distilled water (DDW).
Antibody concentration after reconstitution1 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background

Inositol 1,4,5-trisphosphate receptors (IP₃Rs) are intracellular Ca²⁺ release channels located on the endoplasmic reticulum (ER) and mediate Ca²⁺ mobilization from the ER to the cytoplasm in response to the binding of the second messenger, inositol 1,4,5-trisphosphate (IP₃)¹. IP₃-induced Ca²⁺ release is triggered by various external stimuli, and most non-excitable cells use this mechanism as the primary Ca²⁺signaling pathway. IP₃Rs are therefore thought to have important physiological roles in various cell types and tissues².

Three subtypes of IP₃Rs, derived from three distinct genes, have been identified in mammals³. All three receptors have six transmembrane domains and a pore domain between TM5 and TM6. The N-terminus as well as the C-terminus are cytoplasmic. Each IP₃R consists of an N-terminal ligand binding domain (LBD) and a C-terminal domain which is linked by a long regulatory domain. The C-terminus is constitutively active, suggesting that the regulatory domain is required to maintain the suppression of channel activity⁴. 

Type 2 IP₃R (IP₃R2) is expressed in various tissues and cell lines. IP₃R2 mRNA is localized in the intralobular duct cells of the submandibular gland, the urinary tubule cells of the kidney, the epithelial cells of epididymal ducts and the follicular granulosa cells of the ovary⁵. IP₃R2 is active during osteoclast differentiation, and its absence causes a partial defect in osteoclastic differentiation.

References

1. Furuichi, T.  et al. (1989) Nature 342, 32.
2. Berridge, M.J. et al. (2003) Nat. Rev. Mol. Cell Biol. 4, 517.
3. Furuichi, T. et al. (1994) Curr. Opin. Neurobiol. 4, 294.
4. Varnai, P. et al. (2005)  Proc. Natl. Acad. Sci. U.S.A. 102, 7859.
5. Fujino, I. et al. (1995) Cell Tissue Res. 280, 201.
6. Yaroslavskiy, B.B. et al. (2007) J. Cell Sci. 120, 2884.