Anti-Calcium Homeostasis Modul

Anti-Calcium Homeostasis Modulator 1

CalHM1, FAM26C
Cat #: ACC-101
Sizes: 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: IH, WB
May also work in: IC, IFC, IP
Reactivity: H, M, R

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
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Anti-Calcium Homeostasis Modulator 1 (#ACC-101) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CalHM1 from rat, mouse and human samples.

• Applications
• Specifications
• Scientific Background
• Related Products

Western blot

Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5), U-87 MG (lanes 3 and 6) and C6 (lanes 4 and 8) lysates:
1. Anti-Calcium Homeostasis Modulator 1 antibody (#ACC-101), (1:200).
2. Anti-Calcium Homeostasis Modulator 1 antibody, preincubated with the control peptide antigen.
Immunohistochemistry

Expression of Calcium Homeostasis Modulator 1 in rat hippocampus
Immunohistochemical staining of rat hippocampus using Anti-Calcium Homeostasis Modulator 1 antibody (#ACC-101).  A. Staining of Calcium Homeostasis Modulator 1 appears only in the hippocampal CA1 pyramidal layer (red).  B. Nuclear staining using DAPI as the counterstain (blue).  C. Merge of A and B.
Immunogen
Peptide (C)EAMNHDLELGHTHG, corresponding to amino acid residues 252-265 of human calcium homeostasis modulator protein 1 (Accession Q8IU99). Intracellular, C-terminus.

HomologyRat, mouse - identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.8 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background

A misbalance in Ca²⁺ homeostasis seems to be related to the development of Alzheimer’s disease (AD). Indeed Neuronal Ca²⁺ balance may affect the levels of proteins associated with AD, such as amyloid-beta (Ab) and tau¹.

Calcium homeostasis modulator 1 (CALHM1) is a cerebral Ca²⁺ channel component responsible for controlling intracellular Ca²⁺ levels and Ab metabolism^2,3. CALHM1 is a three transmembrane glycoprotein, mostly localized to the endoplasmic reticulum (ER) although it can be detected at the plasma membrane. A functional channel is formed by multiple subunits which has some structural similarities to the NMDA receptor. It is expressed in all brain regions¹.

In cultured cells, overexpression of CALHM1 increases intracellular Ca²⁺ levels and reduces Ab accumulation, thereby reinforcing its implication in the development of AD. Indeed, a polymorphism in the gene was found to affect the onset of AD².

References

1. Green, K.N. and LaFerla, F.M. (2008) Neuron 59, 190.
2. Koppel, J. et al. (2011) Mol. Med. 17, 974.
3. Dreses-Werringloer, U. et al. (2008) Cell 133, 1149.