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- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 保质期:
1年
- 英文名:
sFas Receptor Human Recombinant
- 库存:
大量
- 供应商:
上海沪震实业有限公司
- 规格:
5μg/20μg/1mg
Lyophilized FAS although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FAS should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
PURITY
Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
AMINO ACID SEQUENCE
MRLSSKSVNA QVTDINSKGL ELRKTVTTVE TQNLEGLHHD GQFCHKPCPP GERKARDCTV NGDEPDCVPC QEGKEYTDKA HFSSKCRRCR LCDEGHGLEV EINCTRTQNT KCRCKPNFFC NSTVCEHCDP CTKCEHGIIK ECTLTSNTKC KEEGSRS.BIOLOGICAL ACTIVITY
The ED50 was determined by its ability to inhibit the cytotoxicity of Jurkat cells is between 10-15 µg/ml in the presence of 2ng/ml of hFasL.USAGE
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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文献和实验相关专题 细胞凋亡专题 Fas/Fas L信号通路 最早被证明在细胞凋亡 中起着重要的作用。Fas是一种跨膜蛋白,属于肿瘤坏死因子受体超家族成员,它与FasL结合可以启动凋亡信号的转导引起细胞凋亡 。它的活化包括一系列步骤:首先配体诱导受体三聚体化,然后在细胞膜 上形成凋亡诱导复合物,这个复合物中包括带有死亡结构域的Fas相关蛋白FADD。Fas一旦和配体FasL结合,可通过Fas分子 启动致死性信号转导,最终
Fas(CD95/APO-1)分子已被证实是一种细胞表面的受体,可与Fas配体(FasL)结合,介导细胞凋亡。本法利用抗-Fas-生物素通过蛋白印迹法、流式细胞分析或免疫组化染色检测Fas/Apo-1分子。 【样本来源】 (1) 单层粘附细胞; (2) 细胞悬液; (3) 组织切片细胞提取物。 【材料与试剂】(Roche公司试剂盒) (1) 鼠抗人-Fas-Biotin; (2) 封闭缓冲液: 0.1mol/L Tris
Visualization of Fas-Mediated Death-Inducing Signaling Complex Formation by Immunoprecipitation
level known as the death-inducing signaling complex (DISC). Here, we describe an immunoprecipitation-based protocol to study DISC assembly in activated human T lymphocytes. This procedure is a useful tool to visualize proteins associated with Fas.
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