- $595
- 丹麦BioPorto Diagnostics A/S
- 丹麦
- JST 001-38
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4-8ºC without exposure to light. No precautions necessary during handling.
- 保质期：
12个月
- 库存：
大量
- 供应商：
上海信裕生物科技有限公司
- 抗体英文名：
APC-PCI Complex specific antibody
- 规格：
200ug/1mg
APC-PCI Complex specific antibody
- Cat. No.
- JST 001-38
- Product name
- APC-PCI Complex specific antibody
- Description
-
Mouse monoclonal antibody
- Specificity
-
JST 001-38 is specific for APC:PCI complex and cleaved PCI but has little reactivity towards native PCI. The dissociation constants (as determined by standard surface plasmon resonance technique) are: Kd= 4x10e-10 M for PCI in complex with APC and 2x10e-10 M for cleaved PCI. The value of Kd for native PCI is too low to measure, i.e. > 10e-5 M.
- Immunogen
-
Mixture of PCI in complex with PSA (approx. 80%) and PCI cleaved from such a complex (approx. 20%).
- Application
- ELISA, WB (Not applicable)
- Species reactivity (positive)
- Human
- Species reactivity (negative)
- Not determined
- Subclass
-
IgG1/k
- Gene ID
- 5104
- Clone number
- M36
- Epitope
-
The epitope is loctated in the calcium-binding N-terminal EGF domain of protein C.
- Content
-
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Protein-A purified
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
-
Activated Protein C (APC), a serine proteinase is inhibited by Protein C inhibitor (PCI). PCI belongs to a group of inhibitors sometimes referred to as serpines (serine proteinase inhibitors), and form 1:1 complexes with APC. PCI plasma concentration is approximately 4ug/m1 .The complex formation between APC and PCI proceeds at a slow rate, which is manifested by a long half-life for APC in plasma, wherein t1/2 is about 20 min.However, the rate of complex formation between APC and PCI is increased by heparin.Upon complex formation with APC, the serpin is cleaved in its so-called bait region, whereby a stable intermediate acyl complex is formed. With time, the intermediate acyl complex dissociates, whereby APC is regenerated and a proteolytically modified, i. e. cleaved, inactive serine proteinase inhibitor is formed.
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文献和实验后期促进复合物(anaphase-promoting complex,APC)
APC即遍在蛋白连接酶(ubiquitin ligase, E3)复合物。 E3通常是一种复合体,由多亚基组成。例如从非洲爪蟾卵细胞中分离的周期蛋白B的E3至少含有8个不同的亚基。APC激发E2-遍在蛋白复合物同有丝分裂周期蛋白破坏框结合, 然后激发遍在蛋白同破坏框C-末端的赖氨酸残基结合，此过程不断循环使遍在蛋白多聚化。
利用RACE [Rapid Amplification of cDNA ENDs，cDNA末端的快速扩增]，可以很简单地得到mRNA的5＇末端序列，然而5＇RACE是一个很复杂的技术，它的成功依赖每一步反应的有效完成。cDNA第一链的合成是由一个反义的基因特异性引物（Gene－specific primer，GSP）来起始的，cDNA第一链纯化后，利用末端脱氧核苷酸转移酶（Terminal deoxynucleotidyl transferase，TdT）在cDNA的5＇末端加上一个合成
Purification of the Mitotic Checkpoint Complex, an Inhibitor of the APC/C From HeLa Cells
The mitotic checkpoint prevents cells with unaligned chromosomes from prematurely exiting mitosis by inhibiting the anaphase-promoting complex/cyclosome (APC/C) from targeting key proteins for ubiquitin-mediated proteolysis. Great efforts
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