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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
见官方网站
- 亚型:
见官方网站
- 形态:
冻干粉或液体
- 保存条件:
-20ºC
- 克隆性:
多克隆
- 标记物:
见官方网站
- 适应物种:
见官方网站
- 保质期:
1年
- 抗原来源:
见官方网站
- 目录编号:
ABS 051-046
- 级别:
见官方网站
- 库存:
30
- 供应商:
上海泽叶生物科技有限公司
- 宿主:
Human
- 应用范围:
ELISA, WB
- 浓度:
1 mg/ml
- 靶点:
见官方网站
- 抗体英文名:
Anti-PYY(3-36) (peptide tyrosine-tyrosine amide, 3-36)
- 抗体名:
Anti-PYY(3-36) (peptide tyrosine-tyrosine amide, 3-36)
- 规格:
200ug
Anti-PYY(3-36) (peptide tyrosine-tyrosine amide, 3-36)
Bioporto是一家体外诊断公司,专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。官方网站:www.bioporto.com
- Cat. No.
- ABS 051-046
- Product name
- Anti-PYY(3-36) (peptide tyrosine-tyrosine amide, 3-36)
- Description
- Mouse monoclonal antibody
- Specificity
- Peptide tyrosine-tyrosine amide (3-36), or PYY(3-36), is a major metabolite of the gut hormone PYY and is produced by the action of dipeptidyl peptidase IV on PYY in the intestinal brush border and in the circulation. PYY(3-36) is a potent inhibitor of food intake in rats and humans, acting selectively on the Y2 receptor to inhibit orexigenic NPY neurons in the arcuate nucleus, thus disinhibiting anorexigenic POMC neurons. The anorexigenic effect of PYY(3-36) is additive to that of the co-secreted gut hormone GLP-1.
- Immunogen
- Synthetic N-terminal fragment of human PYY(3-36) coupled to carrier
- Application
- ELISA, WB
- Species reactivity (positive)
- Human
- Species reactivity (negative)
- Mouse, rat, dog, pig
- Subclass
- IgG1/k
- Gene ID
- 5697
- Clone number
- 04
- Epitope
- The epitope lies within residues 3-12 of human PYY
- Content
- Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Protein-A purified
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
- Peptide tyrosine-tyrosine amide (3-36), or PYY(3-36), is a major metabolite of the gut hormone PYY and is produced by the action of dipeptidyl peptidase IV on PYY in the intestinal brush border and in the circulation. PYY(3-36) is a potent inhibitor of food intake in rats and humans, acting selectively on the Y2 receptor to inhibit orexigenic NPY neurons in the arcuate nucleus, thus disinhibiting anorexigenic POMC neurons. The anorexigenic effect of PYY(3-36) is additive to that of the co-secreted gut hormone GLP-1.
- Application 1
-
ELISA
ABS 051-04 binds free PYY(3-36) in solution. An ELISA dilution guideline of 1/20,000 applies to PYY(3-36) coated directly at 1 µg/mL.
ABS 051-04 as capture antibody forms a sandwich ELISA antibody pair with biotinylated ABS 029-01 as detection antibody for measuring PYY(3-36).
The calibration curve of a sandwich assay for PYY using ABS 051-04 as the capture antibody and ABS 029-01B as the biotinylated detection antibody. - Application 2
-
WB
ABS 051-04 can be used in Western blotting.
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文献和实验erythrocytes to microvascular endothelial cells CD36 deficiency frequent in sub-Saharan Africa, Asia; associated surprisingly with susceptibility to severe cerebral malaria Development of anti-CD36 antibodies can cause serious complications
36 Large-Scale Extraction of Proteins
Purification of proteins using aqueous two-phase systems (ATPS) has been successfully carried out on large scale for more than a decade (1 –17 ). One of the major advantages of ATPS is that the scaling up is possible through the use
人可溶性CD36 分子 ( sCD36 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 sCD36 单抗包被于酶标板上,标准品和样品中的 sCD36与单抗结合,加入生物素化的抗人 sCD36 ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入底物工作液显蓝
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