Peptide tyrosine-tyrosine amide (3-36), or PYY(3-36), is a major metabolite of the gut hormone PYY and is produced by the action of dipeptidyl peptidase IV on PYY in the intestinal brush border and in the circulation. PYY(3-36) is a potent inhibitor of food intake in rats and humans, acting selectively on the Y2 receptor to inhibit orexigenic NPY neurons in the arcuate nucleus, thus disinhibiting anorexigenic POMC neurons. The anorexigenic effect of PYY(3-36) is additive to that of the co-secreted gut hormone GLP-1.
Immunogen
Synthetic N-terminal fragment of human PYY(3-36) coupled to carrier
Application
ELISA, WB
Species reactivity (positive)
Human
Species reactivity (negative)
Mouse, rat, dog, pig
Subclass
IgG1/k
Gene ID
5697
Clone number
04
Epitope
The epitope lies within residues 3-12 of human PYY
Content
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Peptide tyrosine-tyrosine amide (3-36), or PYY(3-36), is a major metabolite of the gut hormone PYY and is produced by the action of dipeptidyl peptidase IV on PYY in the intestinal brush border and in the circulation. PYY(3-36) is a potent inhibitor of food intake in rats and humans, acting selectively on the Y2 receptor to inhibit orexigenic NPY neurons in the arcuate nucleus, thus disinhibiting anorexigenic POMC neurons. The anorexigenic effect of PYY(3-36) is additive to that of the co-secreted gut hormone GLP-1.
Application 1
ELISA
ABS 051-04 binds free PYY(3-36) in solution. An ELISA dilution guideline of 1/20,000 applies to PYY(3-36) coated directly at 1 µg/mL.
ABS 051-04 as capture antibody forms a sandwich ELISA antibody pair with biotinylated ABS 029-01 as detection antibody for measuring PYY(3-36).
The calibration curve of a sandwich assay for PYY using ABS 051-04 as the capture antibody and ABS 029-01B as the biotinylated detection antibody.