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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
见官方网站
- 亚型:
见官方网站
- 形态:
冻干粉或液体
- 保存条件:
-20ºC
- 克隆性:
多克隆
- 标记物:
见官方网站
- 适应物种:
见官方网站
- 保质期:
1年
- 抗原来源:
见官方网站
- 目录编号:
HYB 238-042
- 级别:
见官方网站
- 库存:
30
- 供应商:
上海泽叶生物科技有限公司
- 宿主:
Human
- 应用范围:
ELISA, WB, IHC-P
- 浓度:
1 mg/ml
- 靶点:
见官方网站
- 抗体英文名:
Anti-Surfactant protein A (human)
- 抗体名:
Anti-Surfactant protein A (human)
- 规格:
200ug
Anti-Surfactant protein A (human)
Bioporto是一家体外诊断公司,专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。官方网站:www.bioporto.com
- Cat. No.
- HYB 238-042
- Product name
- Anti-Surfactant protein A (human)
- Description
- Mouse monoclonal antibody
- Specificity
- HYB 238-04 is specific for human pulmonary Surfactant protein A (SP-A).
- Immunogen
- Native SP-A purified from bronchioaveolar lavage from proteinosis patients
- Application
- ELISA, WB, IHC-P
- Species reactivity (positive)
- Human
- Species reactivity (negative)
- Not determined
- Subclass
- IgG1/k
- Gene ID
- 653509
- Clone number
- 6F10
- Epitope
- Not determined
- Content
- Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Protein-A purified
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
- Surfactant protein A (SP-A) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular 'head' regions linked by triple-helical, collagen-like, strands. This group also includes SP-D and the serum proteins mannan-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages. The serum concentration is 45 ng/ml in health
- Application 1
-
ELISA
HYB 238-04 reacts strongly with SP-A when tested in ELISA with SP-A directly coated onto the microtiter well. - Application 2
-
WB
In Western blotting after SDS-PAGE HYB 238-04 reacts with reduced forms of SP-A and weaker with non-reduced forms. A dilution guideline of 1/1000 has proved successful (2). - Application 3
-
IHC
In immunohistochemical staining from paraffin embedded tissue samples, HYB 238-04 reacts specifically with human SP-A. Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages. No reactivity in samples from pancreas, intestines, thymus, prostate gland or the peritoneal cavity lining cells (1, 2).


Paraffin-embedded sections of human lung immunostained with HYB 238-04 (1)
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文献和实验a simple ELISA method to detect human anti-antibody response.
Surfactant-Based Methods for Prevention of Protein Adsorption in Capillary Electrophoresis
Surfactants such as didodecyldimethyl ammonium bromide (DDAB) and 1,2-dilauroyl-sn -phosphatidylcholine (DLPC) form bilayers at the walls of bare silica capillaries. Once formed, these bilayers are stable in the absence of surfactant
Immunohistochemistry: Mouse Anti-Human MCP-1
. 4. Incubate the tissue section overnight at 4?C with Mouse Anti-Human MCP-1 at 10.0 μg/mL. Wash the slide twice for three minutes each. 5. Incubate the tissue section with the Envision HRP polymer kit (Dako) according to the manufacturer’s
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