- $410
- BioPorto
- 丹麦
- HYB 109-031
- 2025年07月05日
- ELISA, WB
- Human
- 见官方网站
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
见官方网站
- 亚型：
见官方网站
- 形态：
冻干粉或液体
- 保存条件：
-20ºC
- 克隆性：
多克隆
- 标记物：
见官方网站
- 适应物种：
见官方网站
- 保质期：
1年
- 抗原来源：
见官方网站
- 目录编号：
HYB 109-031
- 级别：
见官方网站
- 库存：
30
- 供应商：
上海泽叶生物科技有限公司
- 宿主：
Human
- 应用范围：
ELISA, WB
- 浓度：
1 mg/ml
- 靶点：
见官方网站
- 抗体英文名：
Anti-Thrombin (human)
- 抗体名：
Anti-Thrombin (human)
- 规格：
200ug
Anti-Thrombin (human)
Bioporto是一家体外诊断公司，专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。官方网站：www.bioporto.com
- Cat. No.
- HYB 109-031
- Product name
- Anti-Thrombin (human)
- Description
- Mouse monoclonal antibody
- Specificity
- HYB 109-03 binds to human thrombin and to complex of human thrombin with the thrombin inhibitor hirudin. No cross-reaction is seen with prothrombin.
- Immunogen
- Thrombin isolated from activated human plasma
- Application
- ELISA, WB
- Species reactivity (positive)
- Human
- Species reactivity (negative)
- Not determined
- Subclass
- IgG1/k
- Gene ID
- 2147
- Clone number
- 5D10
- Epitope
- Not determined
- Content
- Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Protein-A purified
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
- Thrombin is the active enzyme that splits fibrinogen into fibrin in final stage of blood coagulation. It is generated from its circulating inactive precursor, prothrombin, by the action of factor Xa aided by factor Va, phospholipid and calcium ions. Thrombin is a glycoprotein formed by a 6-kDa A chain linked by a disulfide bridge to a 31-kDa B chain which carries the serine protease catalytic site.
- Application 1
-
ELISA
HYB 109-03 reacts strongly in ELISA with thrombin coated directly onto the microtiter wells. Biotinylation of thrombin blocks antibody binding. - Application 2
-
WB
HYB 109-03 can be used in Western blotting.
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文献和实验a simple ELISA method to detect human anti-antibody response.
Generation of thrombin has been established as the critical process leading to coagulation in vivo. Indeed, ex vivo markers of thrombin generation in patients have been useful in detecting thrombosis, while many standard global clot-time
Immunohistochemistry: Mouse Anti-Human MCP-1
. 4. Incubate the tissue section overnight at 4?C with Mouse Anti-Human MCP-1 at 10.0 μg/mL. Wash the slide twice for three minutes each. 5. Incubate the tissue section with the Envision HRP polymer kit (Dako) according to the manufacturer’s
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