- $940
- BioPorto
- 丹麦
- BW 014
- 2025年07月15日
- ELISA
- 见官方网站
- 见官方网站
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
见官方网站
- 亚型：
见官方网站
- 形态：
冻干粉或液体
- 保存条件：
-20ºC
- 克隆性：
多克隆
- 标记物：
见官方网站
- 适应物种：
见官方网站
- 保质期：
1年
- 抗原来源：
见官方网站
- 目录编号：
BW 014
- 级别：
见官方网站
- 库存：
30
- 供应商：
上海泽叶生物科技有限公司
- 宿主：
见官方网站
- 应用范围：
ELISA
- 浓度：
1 mg/ml
- 靶点：
见官方网站
- 抗体英文名：
C-terminal specific "Active GLP-1" Matched Antibody Pair
- 抗体名：
C-terminal specific "Active GLP-1" Matched Antibody Pair
C-terminal specific "Active GLP-1" Matched Antibody Pair
Bioporto是一家体外诊断公司，专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。官方网站：www.bioporto.com
- Cat. No.
- BW 014
- Product name
- C-terminal specific "Active GLP-1" Matched Antibody Pair
- Description
-
Mouse monoclonal antibodies
- Specificity
-
This uses HYB 147-06 as capture antibody in combination with biotinylated detection antibody ABS 033-10B exclusively to measure GLP-1(7-36)amide in biological samples. GLP-1(7-36)amide is the principal active form of the peptide, and has been estimated to make up about 70% of the physiological response. Relative to measuring both active forms of GLP-1 together, the responses are reduced by not measuring the approximately 30% contribution of GLP-1(7-37) to the overall response. There is no cross-reactivity with any extended or truncated physiological form of GLP-1(7-36)amide.
HYB 147-06-02 495 € ABS 033-10B-005 380 € Total 875 € Matched Pair Price 785 €
- Immunogen
-
Synthetic GLP-1 (7-17)
- Application
- ELISA
- Content
-
200 µL capture antibody and 50 µl detection antibody. 1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Target
-
Glucagon-like peptide 1(7-36)amide (GLP-1(7-36)amide) is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.
- Application 1
-
ELISA
HYB 147-06 (as capture antibody) forms a sandwich ELISA pair with ABS 033-10B (as biotinylated detection antibody) for measuring the principal active form of GLP-1.
This uses HYB 147-06 as capture antibody in combination with biotinylated detection antibody ABS 033-10 exclusively to measure GLP-1(7-36)amide in biological samples. GLP-1(7-36)amide is the principal active form of the peptide, and has been estimated to make up about 70% of the physiological response. Relative to measuring both active forms of GLP-1 together, the responses are reduced by not measuring the approximately 30% contribution of GLP-1(7-37) to the overall response. There is no cross-reactivity with any extended or truncated physiological form of GLP-1(7-36)amide. Specimens must be collected into tubes containing an adequate amount of DPP-4 inhibitor (in addition to routine peptidase inhibitors) to prevent therapid in vitro degradation of the active peptide.
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文献和实验Identification of a Mutant Kinase/ATP Analog Pair
available phospho-specific antibody to the ERK2 substrate Elk1. A phospho-specific antibody allows a large number of ATP analogs to be screened without their having to be made radioactive. In this assay, active wild-type or mutant kinase is mixed with ATP
and rinse once with PBS. Remove the PBS and immediately add -20°C methanol. (Do not allow the cells to dry.) Put the plate in a -20°C freezer for 5 min. Remove the methanol and add PHEM buffer. Fixed cells are kept at 4°C in PHEM. C. Antibody
immunoprecipitation (ChIP) is a powerful and widely applied technique for detecting the association of individual proteins with specific genomic regions in vivo. Live cells are treated with formaldehyde to generate protein?protein and protein? DNA cross?links
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