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- 供应商:
江蓝纯
- 规格:
2ml
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| P2006 | Protein A Agarose (Fast Flow, 进口分装) | 2ml | 439.00元 |
本Protein A Agarose (Fast Flow)为进口分装,主要用于免疫沉淀(Immunoprecipitation,IP)或免疫共沉淀(Co-IP),也可以用于抗体的纯化。
Protein A Agarose适合于免疫沉淀mouse IgG2a, IgG2b, IgA, rabbit IgG, 以及human IgG1, IgG2和IgG4。
Protein A共价交联到6% agarose beads (Fast Flow)上,2ml Protein A Agarose中共含有约2.5mg重组的Protein A。2 ml Protein A Agarose共可以结合约20mg human IgG。推荐的线性流速(Linear flow rate)为 50-300cm/h。
Protein A Agarose配制在TBS溶液中,2ml中共含有0.5ml Agarose beads。
本Protein A Agarose如果用于常规的免疫沉淀,可以免疫沉淀100次。
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文献和实验Fast and reliable mini-prep RNA extraction from Neurospora crassa
of any protein or polysaccharide contamination. Usually, traces of DNA present in RNA preparations can be seen in the slots of ethidium bromide stained formaldehyde gels. Based on this criterion, RNA samples prepared by the described method were free
Analysis of Protein-DNA Binding by Streptavidin-Agarose Pulldown
expressions are regulated. Commonly used techniques to determine DNA-protein binding such as the electrophoretic mobility shift assay (EMSA) have limited value for analyzing simultaneously a large number of proteins in the complex
Use of Flow Cytometric Methods to Quantify Protein‐Protein Interactions
for the quantitative analysis of protein?protein interactions using the flow cytometry protein interaction assay (FCPIA). This method is based upon immobilizing protein on a polystyrene bead, incubating these beads with a fluorescently labeled binding partner
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