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-20℃
6个月
pDONR-P2RP3
大量
钰博生物
1ug/5ug
出品公司: | Ybscience |
---|---|
载体名称: | pDONR-P2RP3 、pDONR P2R-P3 |
质粒类型: | Gateway载体;DONR载体 |
高拷贝/低拷贝: | 低拷贝 |
启动子: | 无 |
克隆方法: | Gateway;BP反应 |
载体大小: | 4773bp |
5' 测序引物及序列: | M13/pUC fwd 5'CCCAGTCACGACGTTGTAAAACG 3' |
3' 测序引物及序列: | M13/pUC rev 5' AGCGGATAACAATTTCACACAGG 3' |
载体标签: | 无 |
载体抗性: | 卡那霉素,氯霉素(仅空载体) |
真核筛选标记: | 无 |
克隆菌株: | 阳性筛选用DB3.1 ,阴性筛选用OmniMAX2-T1 |
备注: | pDONR-P2RP3载体是MultiSite Gateway载体, 与pDONR221、pDONR-P4P1R载体一同构成三片段系统, 目标载体是pDEST-R4R3,是研究启动子的理想工具。 |
病毒/非病毒: | 非病毒 |
LOCUS Exported 4773 bp ds-DNA circular SYN 10-AUG-2016 .KEYWORDS pDONR-P2RP3REFERENCE 1 (bases 1 to 4773) AUTHORS Biofeng Lab.FEATURES Location/Qualifiers source 1..4773 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 268..295 /note="rrnB T2 terminator" /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /note="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 537..553 /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" protein_bind complement(591..822) /gene="mutant version of attP" /bound_moiety="BP Clonase(TM)" /note="attP2" /note="recombination site for the Gateway(R) BP reaction (pDONR(TM)201 version)" promoter 980..1082 /note="cat promoter" /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 1083..1742 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /note="CmR" /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" CDS 2084..2389 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /note="ccdB" /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" protein_bind complement(2746..2977) /gene="mutant version of attP" /bound_moiety="BP Clonase(TM)" /note="attP3" /note="recombination site for the Gateway(R) BP reaction" promoter complement(3015..3033) /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3038..3054) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" CDS 3167..3976 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /note="KanR" /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" rep_origin 4123..4711 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac 601 taataccatc taagtagttg attcatagtg actgcatatg ttgtgtttta cagtattatg 661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt 721 ctcgttcaac tttcttgtac aaagttggca ttataaaaaa gcattgctta tcaatttgtt 781 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttggagctct agagcgtcga 841 ctaagttggc agcatcaccc gacgcacttt gcgccgaata aatacctgtg acggaagatc 901 acttcgcaga ataaataaat cctggtgtcc ctgttgatac cgggaagccc tgggccaact 961 tttggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac cataatgaaa 1021 taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta 1081 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag 1141 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg 1201 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta 1261 ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg 1321 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg 1381 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat 1441 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg 1501 agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg 1561 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag 1621 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt gatggcttcc 1681 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt 1741 aatcgcgtgg atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt 1801 tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtgtgc 1861 tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata 1921 tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg 1981 cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt 2041 gaaatgaacg gctcttttgc tgacgagaac agggactggt gaaatgcagt ttaaggttta 2101 cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga 2161 cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt 2221 ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac 2281 cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg 2341 cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc 2401 ccttatacac agccagtctg caggtcgata cagtagaaat tacagaaact ttatcacgtt 2461 tagtaagtat agaggctgaa aatccagatg aagccgaacg acttgtaaga gaaaagtata 2521 agagttgtga aattgttctt gatgcagatg attttcagga ctatgacact agcgtatatg 2581 aataggtaga tgtttttatt ttgtcacaca aaaaagaggc tcgcacctct ttttcttatt 2641 tctttttatg atttaatacg gcattgagga caatagcgag taggctggat acgacgattc 2701 cgtttgagaa gaacatttgg aaggctgtcg gtcgagctcg aattctacag gtcactaata 2761 ccatctaagt agttgattca tagtgactgc atatgttgtg ttttacagta ttatgtagtc 2821 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt 2881 tcaactttat tatacaaagt tggcattata aaaaagcatt gcttatcaat ttgttgcaac 2941 gaacaggtca ctatcagtca aaataaaatc attatttgga gctccatggt agcgttaacg 3001 cggccgcgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct 3061 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg 3121 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca 3181 acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa 3241 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc 3301 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga 3361 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt 3421 tatccgtact cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt 3481 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt 3541 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt 3601 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga 3661 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc 3721 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga 3781 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca 3841 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct 3901 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct 3961 cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct 4021 gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt 4081 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 4141 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 4201 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 4261 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 4321 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 4381 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 4441 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 4501 cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 4561 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 4621 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 4681 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 4741 cctggccttt tgctggcctt ttgctcacat gtt
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