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-20℃
6个月
pCMV-RFP-C-Myc
大量
钰博生物
1ug/5ug
出品公司: | Ybscience |
---|---|
载体名称: | pCMV-RFP-C-Myc |
质粒类型: | 哺乳动物表达载体;荧光报告载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | CMV |
载体大小: | 4726 bp |
5' 测序引物及序列: | CMV fwd 5’CGCAAATGGGCGGTAGGCGTG 3’ |
3' 测序引物及序列: | BGH rev 5''TAGAAGGCACAGTCGAGG3'' |
载体标签: | RFP、Myc (C ter) |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | DH5α 等 |
宿主细胞(系): | 哺乳动物细胞 |
备注: | -- |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
LOCUS Exported 4726 bp ds-DNA circular SYN 12-AUG-2016KEYWORDS pCMV-RFP-C-MycSOURCE synthetic DNA construct ORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 4726) AUTHORS Biofeng Lab.FEATURES Location/Qualifiers source 1..4726 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 67..370 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 371..618 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" promoter 625..643 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 650..652 /note="ATG" misc_feature 659..696 /note="MCS" gene 707..1399 /note="RFP" CDS 1400..1429 /codon_start=1 /product="Myc (human c-Myc oncogene) epitope tag" /note="Myc" /translation="EQKLISEEDL" polyA_signal 1459..1584 /note="bGH poly(A)" /note="bovine growth hormone polyadenylation signal" rep_origin complement(1642..2097) /direction=LEFT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 2124..2228 /gene="bla" /note="AmpR promoter" promoter 2328..2587 /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 2438..2573 /note="SV40 ori" /note="SV40 origin of replication" CDS 2622..3416 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 3648..3695 /note="HSV TK poly(A)" /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4001..4644 /direction=RIGHT /note="pUC ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"ORIGIN 1 tagttattaa tcccgggagt aatcaattac ggggtcatta gttcatagcc catatatgga 61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 541 gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc 601 ttactggctt atcgaaattc tagataatac gactcactat agggcgaata tggccacggg 661 cgcgccctgc aggctagcct cgagggatcc gatatcgcgg ccgcttcttt ccaaacaagt 721 tttgcctcat gatgtgaaaa tgagatacca tatggatgga tgtgttaacg gtcacagttt 781 cactattgag ggagaaggtg ctggtaaacc ttatgaaggc aaaaagatct tggaactgcg 841 tgtaacaaag ggaggaccac ttccattcgc ctttgacatt ttgtccagcg ttttcactta 901 tggtaacagg tgtttctgtg agtatccaga ggatatgccc gactacttta agcaatcatt 961 gcccgagggt cactcttggg agagaacttt aatgtttgag gatggaggat gcggtactgc 1021 ttcagcccat atatcgttgg acaaaaactg tttcgtgcat aagtctacgt ttcatggtgt 1081 gaattttcct gctaatggtc cggtcatgca gaaaaaggca atgaattggg agcctagctc 1141 tgaactgatc accgcatgtg atggtattct caaaggcgac gttaccatgt ttttgttgct 1201 ggaaggtggt cacagattaa agtgccaatt cacaacatcc tacaaggctc ataaagcagt 1261 gaagatgcca ccaaatcaca ttatcgagca tgtcctagtc aaaaaggaag ttgctgacgg 1321 ttttcaaatc caggaacacg ctgttgcaaa acacttcaca gtagatgtta aggaaactcg 1381 cggccgcgaa ttcagatctg agcagaagct gatctccgag gaggacctgg ttaacttatg 1441 ataaggccgg cctgatcaaa aatcagcctc gactgtgcct tctagttgcc agccatctgt 1501 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc 1561 ctaataaaat gaggaaattg catctgtaca tctagcattc agttgtggtt tgtccaaact 1621 catcaattta tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa 1681 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata 1741 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac 1801 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc 1861 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa 1921 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg 1981 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg 2041 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag 2101 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 2161 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 2221 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa 2281 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 2341 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 2401 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 2461 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 2521 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 2581 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat 2641 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac 2701 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc 2761 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc 2821 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag 2881 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc 2941 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg 3001 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc 3061 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc 3121 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga 3181 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca 3241 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg 3301 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg 3361 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg 3421 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga 3481 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg 3541 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct 3601 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag 3661 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg 3721 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc 3781 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg 3841 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact 3901 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 3961 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 4021 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 4081 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 4141 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca 4201 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 4261 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 4321 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 4381 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 4441 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 4501 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 4561 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 4621 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 4681 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat
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