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- 详细信息
- 技术资料
- 保存条件:
冷藏
- 保质期:
长期
- 英文名:
Recombinant Human Fibroblast Growth Factor 21
- 库存:
965
- 供应商:
北京百奥莱博科技有限公司
特别声明:本产品及我公司所售其他产品均为科研类试剂产品,严禁用于药物、医疗及其他非科研用途。
北京百奥莱博主要从事生物试剂的生产和销*(代"售"),产品线涵盖重组人FGF21(成纤维生长因子21)厂家在内的分子生物学试剂产品,还包括PCR及荧光定量PCR、核酸提取试剂盒类、样本核酸保护试剂、核酸提取、克隆与转化、蛋白纯化、蛋白检测、免疫组化、细胞凋亡与抗体等。
名称:重组人FGF21(成纤维生长因子21)厂家
规格:10μg|50μg|500μg|1mg
编号:JN0232
产地:国产|进口
英文名:Recombinant Human Fibroblast Growth Factor 21
品牌:百奥莱博
本品由我们的大肠杆菌表达系统制备,目的基因编码的His29-Ser209在N端含有His标签
FGF-21质量控制:>95%(还原性SDS-PAGE)。
FGF-21制剂:冻干品
FGF-21保存:
冻干蛋白可在-20℃长期保存,室温也可稳定保存3周。
复溶蛋白溶液可在4~7℃保存2~7天。
复溶后建议分装冻存在-20℃,可保存3个月,应避免反复冻融。
FGF-21复溶:
1、试剂开盖前请先离心。
2、复溶浓度应大于100 μg/ml.
3、冻干蛋白溶于ddH2O中
4、复溶后根据使用量分装保存,避免反复冻融。
关于FGF-21:
Fibroblast Growth Factor 21 (FGF21) is a growth factor that belongs to the FGF family. FGF family proteins play a central role during prenatal development and postnatal growth and regeneration of mamy tissues, by promoting cellular proliferation and differentiation. FGF21 is a potent activator of glucose uptake on adipocytes, protects animal from diet-induced obesity when overexpression in transgenic mice, and lower blood glucose and triglyceride levels when therapeutically adiministered to diabetic redents. FGF21 is produced by hepatocytes in reponse to free fatty acid stimulation of a PPARa/RXR dimeric complex. This situation occurs clinically during starvation, or following the ingestionof a highly-fat/low-carbohydrate diet.Upon FGF21 secretion, white adipose tissue is induced to release free fatty acids from triglyceride stores. Once free fatty acid reach hepatocytes, they are oxidized and reduced to acetyl-CoA. The acetyl-CoA is recombined into 4-carbon ketone bodies, release, and transported to peripheral tissue for TCA processing and energy generation.
欲了解更多重组人FGF21(成纤维生长因子21)厂家的品牌、产地、价格及说明书等产品信息,请致电北京百奥莱博科技有限公司,我们将竭诚为您服务,另外,以下产品正在火爆促销:
·重组人uPAR(尿激酶型纤溶酶原激活物受体)
编号:JN1823
英文名称:Recombinant Human Urokinase Plasminogen Activator Surface Receptor
规格:10μg|50μg|500μg|1mg
本品由我们的哺乳动物细胞表达系统制备而成,目的基因编码的Leu23-Arg303在C端含有His标签。
uPAR质量控制:>95%(还原性SDS-PAGE)
uPAR制剂:冻干品
uPAR保存:
冻干蛋白置于-20℃以下可长期保存,室温条件下可稳定保存3周。
复溶蛋白溶液可在4~7℃保存2~7天,可分装后置于-20℃保存三个月。
uPAR复溶:
打开试剂管前请先离心。
复溶浓度推荐大于100 μg/ml。
冻干蛋白请溶于ddH2O。
复溶后,请根据用量分装冻存,避免反复冻融。
关于uPAR:
The Urokinase Type Plasminogen Activator (uPA) receptor (uPAR) is a widely expressed receptor for urokinase plasminogen activator (uPA) and pro-uPA. uPAR / CD87 is a highly glycosylated, 55-60kDa integral membrane protein linked to the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor. uPAR is expressed by T-cells, NK cells, monocytes, and neutrophils as well as non-hematopoietic cells that include vascular endothelial cells, fibroblasts, smooth muscle cells, keratinocytes, placental trophoblasts, hepatocytes, and a wide variety of tumor cells (including breast, colon, and prostate carcinoma, melanoma). It plays a critical role in the regulation of cell-surface plasminogen activation in physiological and pathological conditions, and it is also involved in cellular adhesion, the transmission of extracellular signals across the plasma membrane and the subsequent regulation of gene expression. uPAR has been implicated in several biological processes including angiogenesis, monocyte migration, cancer metastasis, trophoblast implantation, and wound healing. Human uPAR is encoded as a 313 amino acid residue polypeptide, excluding a 22 residue signal peptide and shows 60-70% similarity with the murine uPAR amino acid sequence although binding of uPA to uPAR shows strong species specificity.
重组人FGF21(成纤维生长因子21)厂家关键词:重组人成纤维生长因子21,JN0232,重组人FGF21蛋白,重组FGF21
·Cas9蛋白(Cas9核酸酶)
编号:JN0065
英文名称:Cas9 Protein
规格:50U
CRISPR/Cas9是细菌和古细菌在长期演化过程中形成的一种适应性免疫防御系统。CRISPR/Cas9 系统通过将入侵噬菌体和质粒 DNA 的片段整合到 CRISPR 序列中,并利用相应的 CRISPR RNAs(crRNAs)来指导Cas9蛋白对同源序列的降解,从而提供免疫性。人工改造过的Cas9/sgRNA系统通过sgRNA(short guide RNA)引导Cas9蛋白识别并剪切带有sgRNA靶点的双链DNA,可用于基因敲除和精确编辑DNA等操作。
产品特点:纯蛋白体系,避免CRISPR基因敲除时引入质粒或病毒干扰。
产品用途:
1、基于蛋白与sgRNA转染的非病毒载体CRISPR基因敲除;
2、基于蛋白与sgRNA转染的非病毒载体CRISPR基因敲入;
3、sgRNA剪切靶点DNA的效率检测,降低体内筛选成本;
4、体外剪切靶DNA的特异位点。
应用实例:

图例一)Cas9核酸内切酶 DNA片段切割活性检测。
带有靶位点的DNA片段:6100bp;酶切后的片段:3900bp和2200bp。
活性定义:37℃,在sgRNA足量的20μL反应体系中,1h完全切割1μg质粒或线性化DNA所需要的酶量定义为1个活性单位(U)。
质量保证:经多次柱纯化,SDS-PAGE胶检测仅可见清晰单一的目的条带;PCR方法检测无大肠杆菌DNA残留;无核酸内、外切酶污染;无RNase污染。
体外切割体系(20μl):
| 组分 | 体积 |
| dsDNA | 200nM |
| sgRNA | 100nM |
| Cas9 酶 | 1U |
| 10×Reaction Buffer | 2μL |
| RNase Free Water | To 20μL |
| 37℃反应1h,琼脂糖胶电泳检测 | |
*可在反应体系中添加20U的RNase Inhibitor,防止dsDNA不纯导致sgRNA的降解。
储存条件:-20℃
重组人FGF21(成纤维生长因子21)厂家关键词:重组人成纤维生长因子21,JN0232,重组人FGF21蛋白,重组FGF21
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北京百莱博科技有限公司是分子生物学试剂产品的专业生产供应销*(代"售")商,恭候您的咨询选购重组人FGF21(成纤维生长因子21)厂家。
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