FUW-M2rtTA

FUW-M2rtTA

载体基本信息

出品公司:	Ybscience
载体名称:	FUW-M2rtTA
质粒类型:	慢病毒载体；四环素调控载体
高拷贝/低拷贝:	高拷贝
克隆方法:	限制性内切酶，多克隆位点
启动子:	Ubc
载体大小:	7979 bp
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	--
载体抗性:	氨苄青霉素
筛选标记:	--
克隆菌株:	Stbl3
宿主细胞（系）:	大部分哺乳动物细胞
备注:	FUW-M2rtTA vector expressing the reverse tetracycline transactivator
稳定性:	稳表达
组成型/诱导型:	诱导型
病毒/非病毒:	慢病毒

 

载体质粒图谱和多克隆位点信息

 

载体简介

Lentiviral plasmid expressing the reverse tetracycline transactivator.

载体序列

LOCUS       FUW-M2rtTA	7979 bp 	DNA	SYN
DEFINITION  FUW-M2rtTA
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..7979
                     /organism="FUW-M2rtTA"
                     /mol_type="other DNA"
     misc_feature    7..594
                     /label="WPRE"
     CDS             95..1144
                     /label="ORF frame 2"

     CDS             108..620
                     /label="ORF frame 3"

     misc_feature    complement(597..613)
                     /label="pBluescriptKS_primer"

     misc_feature    781..796
                     /label="cPPT"

     misc_feature    781..802
                     /label="U3PPT"

     misc_feature    1118..1298
                     /label="HIV-1_5_LTR"

     misc_feature    1118..1298
                     /label="truncHIV-1_3_LTR"

     misc_feature    complement(1324..1341)
                     /label="BGH_rev_primer"

     gene            complement(2346..3206)
                     /label="Ampicillin"
                     /gene="Ampicillin"

     CDS             complement(2346..3206)
                     /label="ORF frame 1"

     promoter        complement(3248..3276)
                     /label="AmpR_promoter"

     promoter        3578..4154
                     /label="CMV_immearly_promoter"

     misc_feature    3657..3944
                     /label="CAG_enhancer"

     misc_feature    4111..4131
                     /label="CMV_fwd_primer"

     misc_feature    4174..4354
                     /label="truncHIV-1_3_LTR"

     misc_feature    4174..4354
                     /label="HIV-1_5_LTR"

     misc_feature    4465..4509
                     /label="HIV-1_psi_pack"

     CDS             4897..5784
                     /label="ORF frame 1"

     misc_feature    5019..5252
                     /label="RRE"

     misc_feature    5783..5798
                     /label="cPPT"

     promoter        5956..7172
                     /label="hUbC_promoter"

     CDS             7201..7947
                     /label="ORF frame 1"

ORIGIN
    1 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
   61 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
  121 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
  181 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
  241 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
  301 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
  361 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
  421 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
  481 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
  541 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
  601 ccgtcgacct cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
  661 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
  721 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
  781 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
  841 tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc
  901 agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga
  961 gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca
 1021 tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt
 1081 tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat
 1141 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
 1201 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
 1261 cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat
 1321 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
 1381 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
 1441 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
 1501 gggaggattg ggaagacaat agcaggcatg tgagcaaaag gccagcaaaa ggccaggaac
 1561 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
 1621 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
 1681 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
 1741 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
 1801 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
 1861 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
 1921 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
 1981 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
 2041 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
 2101 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
 2161 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
 2221 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
 2281 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
 2341 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
 2401 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
 2461 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
 2521 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
 2581 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
 2641 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
 2701 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
 2761 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
 2821 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
 2881 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
 2941 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
 3001 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
 3061 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
 3121 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
 3181 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
 3241 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
 3301 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
 3361 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
 3421 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
 3481 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
 3541 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
 3601 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
 3661 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
 3721 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
 3781 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
 3841 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
 3901 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
 3961 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
 4021 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
 4081 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
 4141 aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga
 4201 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
 4261 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
 4321 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
 4381 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
 4441 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
 4501 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
 4561 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
 4621 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
 4681 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
 4741 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
 4801 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
 4861 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
 4921 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
 4981 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
 5041 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
 5101 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
 5161 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
 5221 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
 5281 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
 5341 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
 5401 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
 5461 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
 5521 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
 5581 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
 5641 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
 5701 gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat
 5761 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
 5821 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
 5881 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggttaattaa
 5941 cccgtgtcgg ctccagatct ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc
 6001 cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc
 6061 cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag aaccccagta
 6121 tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg ttttctttcc
 6181 agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg agggatctcc
 6241 gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac agctagttcc
 6301 gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc gtcacttggt
 6361 gagtagcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct cggtgggacg
 6421 gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag gttgccctga
 6481 actgggggtt ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg aatggaagac
 6541 gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga
 6601 acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg
 6661 gggcaccatc tggggaccct gacgtgaagt ttgtcactga ctggagaact cggtttgtcg
 6721 tctgttgcgg gggcggcagt tatggcggtg ccgttgggca gtgcacccgt acctttggga
 6781 gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat gcagggtggg
 6841 gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag ggttcgggcc
 6901 tagggtaggc tctcctgaat cgacaggcgc cggacctctg gtgaggggag ggataagtga
 6961 ggcgtcagtt tctttggtcg gttttatgta cctatcttct taagtagctg aagctccggt
 7021 tttgaactat gcgctcgggg ttggcgagtg tgttttgtga agttttttag gcaccttttg
 7081 aaatgtaatc atttgggtca atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta
 7141 aattctggcc gtttttggct tttttgttag acgaagcttg ggcccgggaa ttaattcacc
 7201 atgtctagac tggacaagag caaagtcata aacggcgctc tggaattact caatggagtc
 7261 ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc
 7321 ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg
 7381 gacaggcatc atacccactt ctgccccctg gaaggcgagt catggcaaga ctttctgcgg
 7441 aacaacgcca agtcattccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat
 7501 ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg
 7561 tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt
 7621 acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca
 7681 cctaccaccg attctatgcc cccacttctg agacaagcaa ttgagctgtt cgaccggcag
 7741 ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag
 7801 ctaaagtgcg aaagcggcgg gccggccgac gcccttgacg attttgactt agacatgctc
 7861 ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat
 7921 tttgaccttg acatgctccc cgggtaacta agtaaggatc aattcgatat caagcttat