- ¥800 - 1200
- Ybscience
- 中国/美国
- YB-0862
- 2025年07月15日
14 年金牌会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pLenti CMV/TO Neo empty (w215-1)
- 库存：
大量
- 供应商：
钰博生物
- 规格：
1ug/5ug
pLenti CMV/TO Neo empty (w215-1)
载体基本信息
| 质粒名称: | pLenti CMV/TO Neo empty (w215-1) |
|---|---|
| 出品公司: | Ybscience |
| 存储实验室: | Eric Campeau |
载体骨架基本信息 |
|
| 载体名称: | p156RRL-sinPPT-CMV-GFP-PRE/Nhe I |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 8186 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
插入基因信息 |
|
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Xho I(未破坏) |
| 3' 克隆位点: | Xba I(未破坏) |
| 5' 测序引物 | CMVforw |
| 3' 测序引物 | |
文献引用方法 |
|
| 材料和方法部分: | pLenti CMV/TO Neo empty (w215-1) was a gift from Eric Campeau (Addgene plasmid # 17485) |
| 参考文献部分: | A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394 |
载体质粒图谱和多克隆位点信息
%E8%BD%BD%E4%BD%93%E5%9B%BE%E8%B0%B1.png)
载体简介
载体序列
LOCUS pLenti CMV/TO Neo empty (w215-1) 8186 bp DNA SYN
DEFINITION pLenti CMV/TO Neo empty (w215-1)
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..8186
/organism="pLenti CMV/TO Neo empty (w215-1)"
/mol_type="other DNA"
promoter 62..90
/label="AmpR_promoter"
gene 132..992
/label="Ampicillin"
/gene="Ampicillin"
CDS 132..992
/label="ORF frame 3"
rep_origin 1147..1766
/label="pBR322_origin"
promoter 2075..2104
/label="lac_promoter"
misc_feature 2118..2140
/label="M13_pUC_rev_primer"
promoter 2139..2157
/label="M13_reverse_primer"
promoter 2174..2193
/label="T3_promoter"
misc_feature 2448..2628
/label="HIV-1_5_LTR"
misc_feature 2448..2628
/label="truncHIV-1_3_LTR"
misc_feature 2736..2780
/label="HIV-1_psi_pack"
CDS 3168..3668
/label="ORF frame 3"
misc_feature 3290..3523
/label="RRE"
CDS 3629..4171
/label="ORF frame 2"
misc_feature 4016..4031
/label="cPPT"
promoter 4149..4653
/label="CMV_immearly_promoter"
misc_feature 4156..4443
/label="CAG_enhancer"
misc_feature 4610..4630
/label="CMV_fwd_primer"
misc_feature 4661..4700
/label="tetO"
misc_feature 4741..4765
/label="attB1"
misc_feature 4834..4858
/label="attB2"
misc_feature 4891..5466
/label="WPRE"
CDS 4979..5512
/label="ORF frame 2"
misc_feature complement(5555..5578)
/label="MSCV_rev_primer"
misc_feature 5952..5971
/label="mPGK_F_primer"
gene 6033..6833
/label="NeoR/KanR"
/gene="NeoR/KanR"
CDS 6033..6836
/label="ORF frame 3"
misc_feature 6910..6925
/label="cPPT"
misc_feature 6910..6931
/label="U3PPT"
misc_feature 6980..7160
/label="HIV-1_5_LTR"
misc_feature 6980..7160
/label="truncHIV-1_3_LTR"
terminator 7235..7354
/label="SV40_PA_terminator"
misc_feature 7323..7342
/label="EBV_rev_primer"
rep_origin 7372..7449
/label="SV40_origin"
misc_feature 7434..7453
/label="SV40pro_F_primer"
promoter complement(7540..7558)
/label="T7_promoter"
promoter complement(7568..7584)
/label="M13_forward20_primer"
misc_feature complement(7577..7599)
/label="M13_pUC_fwd_primer"
misc_feature 7577..7720
/label="lacZ_a"
rep_origin 7746..8052
/label="f1_origin"
ORIGIN
1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag
4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgataagct
4141 tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
4201 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
4261 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
4321 tatCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT
4381 TATGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC
4441 ATCGCTATTA CCATGGTGAT GCGGTTTTGG CAGTACATCA ATGGGCGTGG ATAGCGGTTT
4501 GACTCACGGG GATTTCCAAG TCTCCACCCC ATTGACGTCA ATGGGAGTTT GTTTTGGCAC
4561 CAAAATCAAC GGGACTTTCC AAAATGTCGT AACAACTCCG CCCCATTGAC GCAAATGGGC
4621 GGTAGGCGTG TACGGTGGGA GGTCTATATA AGCAGAGCTC TCCCTATCAG TGATAGAGAT
4681 CTCCCTATCA GTGATAGAGA TCGTCGACTA GTCCAGTGTG GTGGAATTCT GCAGATATCA
4741 ACAAGTTTGT ACAAAAAAGC AGGCTCCACC ATGGGAACCA ATTCAGTCGA CTGGATCCGG
4801 TACCGAATTC GCGGCCGCAC TCGAGATATC TAGACCCAGC TTTCTTGTAC AAAGTGGTTG
4861 ATATCCAGCA CAGTGGCGGC CGCTCGAcaa tcaacctctg gattacaaaa tttgtgaaag
4921 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
4981 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
5041 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
5101 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
5161 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
5221 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
5281 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
5341 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
5401 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
5461 ttgggccgcc tccccgcctg gAATTCTGCA GATATCCGGA CTAGTGATCT AATTCTACCG
5521 GGTAGGGGAG GCGCTTTTCC CAAGGCAGTC TGGAGCATGC GCTTTAGCAG CCCCGCTGGG
5581 CACTTGGCGC TACACAAGTG GCCTCTGGCC TCGCACACAT TCCACATCCA CCGGTAGGCG
5641 CCAACCGGCT CCGTTCTTTG GTGGCCCCTT CGCGCCACCT TCTACTCCTC CCCTAGTCAG
5701 GAAGTTCCCC CCCGCCCCGC AGCTCGCGTC GTGCAGGACG TGACAAATGG AAGTAGCACG
5761 TCTCACTAGT CTCGTGCAGA TGGACAGCAC CGCTGAGCAA TGGAAGCGGG TAGGCCTTTG
5821 GGGCAGCGGC CAATAGCAGC TTTGCTCCTT CGCTTTCTGG GCTCAGAGGC TGGGAAGGGG
5881 TGGGTCCGGG GGCGGGCTCA GGGGCGGGCT CAGGGGCGGG GCGGGCGCCC GAAGGTCCTC
5941 CGGAGGCCCG GCATTCTGCA CGCTTCAAAA GCGCACGTCT GCCGCGCTGT TCTCCTCTTC
6001 CTCATCTCCG GGCCTTTCGA CCTGCAGCCA ATATGGGATC GGCCATTGAA CAAGATGGAT
6061 TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC
6121 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC
6181 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC
6241 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG
6301 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC
6361 TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG
6421 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC
6481 GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC
6541 CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA
6601 CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA
6661 TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG
6721 ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG
6781 CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGGGG
6841 ATCCGTCGAG GGCCCGGTAC ctttaagacc aatgacttac aaggcagctg tagatcttag
6901 ccacttttta aaagaaaagg ggggactgga agggctagct cactcccaac gaagacaaga
6961 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
7021 tggctgccta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
7081 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
7141 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
7201 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
7261 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
7321 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
7381 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
7441 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
7501 ggaggcctag gcttttgcgt cgagacgtac ccaattcgcc ctatagtgag tcgtattacg
7561 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
7621 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
7681 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcgac gcgccctgta
7741 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
7801 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
7861 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
7921 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
7981 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
8041 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
8101 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
8161 acaaaatatt aacgtttaca atttcc
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
Characterization and Validation of Cre‐Driver Mouse Lines
2. (A ) Schematic representation of the CreERT2 transgene used for pronuculei injection (Feil et al., ; Indra et al., ). The pair of primers used for RT‐qPCR amplification of Cre transcripts is located in the β‐globin region between a tissue‐specific promoter X (pX
LINEAGE ANALYSIS USING RETROVIRAL VECTORS
. Moreover, the Xgal product and the X-P/NBT precipitate produced by PLAP often are too dark to allow simultaneous detection of another colored precipitate produced by immunohistochemical detection of a cellular antigen. However, occasionally, this will work (e.g
pmirGLO 哺乳细胞质粒 双荧光分析质粒 pTet-On-Advanced 哺乳细胞质粒 四环素调控系统 pTet-On 哺乳细胞质粒 四环素调控系统 Plenti cmv gfp
技术资料暂无技术资料 索取技术资料
pLenti CMV/TO Neo empty (w215-1)
¥800 - 1200
