pVL1392

pVL1392

载体基本信息

出品公司:	Ybscience
载体名称:	pVL1392
质粒类型:	昆虫表达载体；杆状病毒载体
拷贝数:	高拷贝
克隆方法:	Gateway
启动子:	Polyhedrin
载体大小:	9632 bp
5' 测序引物及序列:	Polyhedrin Forward：5' d(AAA TGA TAA CCA TCT CGC) 3'
3' 测序引物及序列:	Polyhedrin Reverse：5' d(GTC CAA GTT TCC CTG) 3'
载体标签:	无标签
载体抗性:	氨苄青霉素
真核筛选标记:	无
克隆菌株:	推荐E.coli TOP10
宿主细胞（系）:	Sf9、Sf21、High Five细胞系
备注:	相关载体有pAC360, pVL1393
瞬表达/稳表达:	稳表达
组成型/诱导型:	组成型
病毒/非病毒:	杆状病毒

 

载体质粒图谱和多克隆位点信息

载体简介

 

pVL1393载体和pVL1392载体简介

pVL1392 and pVL1393 are 9.8 kb vectors designed for use as baculovirus transfer vectors. These transfer vectors contain recombination sequences which are homologous to sequences in the baculovirus genome.
Upon cotransfection, these sequences undergo homologous recombination resulting in a recombinant viral genome packaged in infectious viral particles.
These vectors are nonfusion vectors, which provide the AcMNPV polyhedrin enhancer-promoter sequences to drive high expression, and a multiple cloning site, conveniently constructed in opposite orientations, for simple subcloning. Translation initiation sequences must be provided by the inserted cDNA.


载体特征

• Multiple cloning site polylinker, positioned in opposite orientations
• Recombination sequences for insertion into the baculovirus genome
• Polyhedrin enhancer-promoter sequences for high expression of recombinant protein

Features for growth and maintenance in E. coli:
• Ampicillin gene for selection
• pMB1 origin of replication from pUC19 for high copy number growth

E. coli Strains
The TOP10 cells are provided as a stab.
TOP10 is provided for growth and maintenance of this plasmid. TOP10 is a recombination-negative strain designed for stable replication of high copy number plasmids. This strain is provided as a convenience for those who do not have access to other E. coli strains. Many E. coli strains are suitable for the growth of this vector including DH5α, DH10, and INVαF'. In general, it is best to grow vectors containing inserts in E. coli strains which are recombination-deficient (recA-).

Genotype of TOP10
F-, mcrA ∆(mrr-hsdRMS-mcrBC) Φ80lacZ∆M15 ∆lacX74 deoR recA1 araD139 ∆(ara-leu)7697 galU galK rpsL endA1 nupG 

 

载体序列

LOCUS       pVL1392	9632 bp 	DNA	SYN
DEFINITION  pVL1392
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..9632
                     /organism="pVL1392"
                     /mol_type="other DNA"
     CDS             76..582
                     /label="ORF frame 1"
     CDS             complement(614..1600)
                     /label="ORF frame 2"
     CDS             complement(1868..2323)
                     /label="ORF frame 2"
     CDS             2662..3294
                     /label="ORF frame 1"
     misc_feature    2844..3990
                     /label="Ac_5_flank"
     CDS             complement(3332..3937)
                     /label="ORF frame 2"
     promoter        3991..4091
                     /label="PH_promoter"
     misc_feature    4017..4034
                     /label="polyhedrin_fwd_primer"
     CDS             4250..4741
                     /label="ORF frame 2"
     misc_feature    complement(4288..4302)
                     /label="polyhedrinn_rev_primer"
     misc_feature    4640..4741
                     /label="3_AcPH"
     misc_feature    complement(4657..4674)
                     /label="Baculovirs_rev_primer"
     misc_feature    4755..6180
                     /label="Ac_3_flank"
     CDS             complement(4771..6024)
                     /label="ORF frame 3"
     misc_feature    complement(4853..5651)
                     /label="r_ORF1629"
     CDS             6397..6987
                     /label="ORF frame 1"
     promoter        complement(7009..7027)
                     /label="M13_reverse_primer"
     misc_feature    complement(7026..7048)
                     /label="M13_pUC_rev_primer"
     promoter        complement(7062..7091)
                     /label="lac_promoter"
     gene            complement(8174..9034)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(8174..9034)
                     /label="ORF frame 2"
     promoter        complement(9076..9104)
                     /label="AmpR_promoter"
     misc_feature    complement(9263..9285)
                     /label="pGEX_3_primer"
     misc_feature    complement(9484..9627)
                     /label="lacZ_a"
     misc_feature    9598..9620
                     /label="M13_pUC_fwd_primer"
     promoter        9613..9629
                     /label="M13_forward20_primer"
ORIGIN
    1 aagctttact cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt
   61 gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt
  121 ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac
  181 gtgactagcg aagaagatgt gtggaccgca gaacagatag taaaacaaaa ccctagtatt
  241 ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg
  301 gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata
  361 gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg
  421 ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg
  481 cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac
  541 aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc
  601 tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta
  661 tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag
  721 gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt
  781 ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca
  841 cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat
  901 ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt
  961 tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg
 1021 tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa
 1081 actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg
 1141 tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt
 1201 gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa
 1261 gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc
 1321 cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga
 1381 cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat
 1441 tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt
 1501 ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat
 1561 cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat
 1621 tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa
 1681 tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc
 1741 ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag
 1801 tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt
 1861 atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc
 1921 cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg
 1981 cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt
 2041 ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg
 2101 cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat
 2161 tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca
 2221 atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca
 2281 gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc
 2341 aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac
 2401 acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg
 2461 atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca
 2521 tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt
 2581 atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
 2641 tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
 2701 agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
 2761 aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
 2821 ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
 2881 aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
 2941 aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
 3001 aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
 3061 aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
 3121 gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
 3181 atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag
 3241 ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
 3301 aaacaattat aaatgctaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
 3361 catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
 3421 aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
 3481 tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
 3541 aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
 3601 tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
 3661 tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
 3721 aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
 3781 acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
 3841 ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
 3901 ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
 3961 atcacaaact ggaaatgtct atcaatatat agttgctgat atcatggaga taattaaaat
 4021 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
 4081 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atcagatctg
 4141 cagcggccgc tccagaattc tagaaggtac ccgggatcct ttcctgggac ccggcaagaa
 4201 ccaaaaactc actctcttca aggaaatccg taatgttaaa cccgacacga tgaagcttgt
 4261 cgttggatgg aaaggaaaag agttctacag ggaaacttgg acccgcttca tggaagacag
 4321 cttccccatt gttaacgacc aagaagtgat ggatgttttc cttgttgtca acatgcgtcc
 4381 cactagaccc aaccgttgtt acaaattcct ggcccaacac gctctgcgtt gcgaccccga
 4441 ctatgtacct catgacgtga ttaggatcgt cgagccttca tgggtgggca gcaacaacga
 4501 gtaccgcatc agcctggcta agaagggcgg cggctgccca ataatgaacc ttcactctga
 4561 gtacaccaac tcgttcgaac agttcatcga tcgtgtcatc tgggagaact tctacaagcc
 4621 catcgtttac atcggtaccg actctgctga agaggaggaa attctccttg aagtttccct
 4681 ggtgttcaaa gtaaaggagt ttgcaccaga cgcacctctg ttcactggtc cggcgtatta
 4741 aaacacgata cattgttatt agtacattta ttaagcgcta gattctgtgc gttgttgatt
 4801 tacagacaat tgttgtacgt attttaataa ttcattaaat ttataatctt tagggtggta
 4861 tgttagagcg aaaatcaaat gattttcagc gtctttatat ctgaatttaa atattaaatc
 4921 ctcaatagat ttgtaaaata ggtttcgatt agtttcaaac aagggttgtt tttccgaacc
 4981 gatggctgga ctatctaatg gattttcgct caacgccaca aaacttgcca aatcttgtag
 5041 cagcaatcta gctttgtcga tattcgtttg tgttttgttt tgtaataaag gttcgacgtc
 5101 gttcaaaata ttatgcgctt ttgtatttct ttcatcactg tcgttagtgt acaattgact
 5161 cgacgtaaac acgttaaata aagcttggac atatttaaca tcgggcgtgt tagctttatt
 5221 aggccgatta tcgtcgtcgt cccaaccctc gtcgttagaa gttgcttccg aagacgattt
 5281 tgccatagcc acacgacgcc tattaattgt gtcggctaac acgtccgcga tcaaatttgt
 5341 agttgagctt tttggaatta tttctgattg cgggcgtttt tgggcgggtt tcaatctaac
 5401 tgtgcccgat tttaattcag acaacacgtt agaaagcgat ggtgcaggcg gtggtaacat
 5461 ttcagacggc aaatctacta atggcggcgg tggtggagct gatgataaat ctaccatcgg
 5521 tggaggcgca ggcggggctg gcggcggagg cggaggcgga ggtggtggcg gtgatgcaga
 5581 cggcggttta ggctcaaatg tctctttagg caacacagtc ggcacctcaa ctattgtact
 5641 ggtttcgggc gccgtttttg gtttgaccgg tctgagacga gtgcgatttt tttcgtttct
 5701 aatagcttcc aacaattgtt gtctgtcgtc taaaggtgca gcgggttgag gttccgtcgg
 5761 cattggtgga gcgggcggca attcagacat cgatggtggt ggtggtggtg gaggcgctgg
 5821 aatgttaggc acgggagaag gtggtggcgg cggtgccgcc ggtataattt gttctggttt
 5881 agtttgttcg cgcacgattg tgggcaccgg cgcaggcgcc gctggctgca caacggaagg
 5941 tcgtctgctt cgaggcagcg cttggggtgg tggcaattca atattataat tggaatacaa
 6001 atcgtaaaaa tctgctataa gcattgtaat ttcgctatcg tttaccgtgc cgatatttaa
 6061 caaccgctca atgtaagcaa ttgtattgta aagagattgt ctcaagctcg ccgcacgccg
 6121 ataacaagcc ttttcatttt tactacagca ttgtagtggc gagacacttc gctgtcgtcg
 6181 acgtacatgt atgctttgtt gtcaaaaacg tcgttggcaa gctttaaaat atttaaaaga
 6241 acatctctgt tcagcaccac tgtgttgtcg taaatgttgt ttttgataat ttgcgcttcc
 6301 gcagtatcga cacgttcaaa aaattgatgc gcatcaattt tgttgttcct attattgaat
 6361 aaataagatt gtacagattc atatctacga ttcgtcatgg ccaccacaaa tgctacgctg
 6421 caaacgctgg tacaatttta cgaaaactgc aaaaacgtca aaactcggta taaaataatc
 6481 aacgggcgct ttggcaaaat atctatttta tcgcacaagc ccactagcaa attgtatttg
 6541 cagaaaacaa tttcggcgca caattttaac gctgacgaaa taaaagttca ccagttaatg
 6601 agcgaccacc caaattttat aaaaatctat tttaatcacg gttccatcaa caaccaagtg
 6661 atcgtgatgg actacattga ctgtcccgat ttatttgaaa cactacaaat taaaggcgag
 6721 ctttcgtacc aacttgttag caatattatt agacagctgt gtgaagcgct caacgatttg
 6781 cacaagcaca atttcataca caacgacata aaactcgaaa atgtcttata tttcgaagca
 6841 cttgatcgcg tgtatgtttg cgattacgga ttgtgcaaac acgaaaactc acttagcgtg
 6901 cacgacggca cgttggagta ttttagtccg gaaaaaattc gacacacaac tatgcacgtt
 6961 tcgtttgact ggtacgcggc gtgttaacat acaagttgct aacgtaatca tggtcatagc
 7021 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
 7081 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
 7141 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
 7201 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
 7261 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
 7321 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
 7381 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
 7441 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
 7501 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
 7561 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
 7621 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
 7681 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
 7741 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
 7801 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
 7861 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
 7921 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
 7981 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
 8041 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
 8101 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
 8161 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
 8221 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
 8281 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
 8341 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
 8401 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
 8461 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
 8521 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
 8581 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
 8641 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
 8701 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
 8761 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
 8821 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
 8881 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
 8941 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
 9001 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
 9061 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
 9121 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
 9181 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
 9241 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
 9301 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
 9361 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
 9421 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
 9481 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
 9541 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
 9601 agtcacgacg ttgtaaaacg acggccagtg cc