YEplac112

YEplac112

载体基本信息

出品公司:	Ybscience
载体名称:	YEplac112
质粒类型:	酿酒酵母表达载体；2μ质粒型载体；酵母附加体型载体
高拷贝/低拷贝:	高拷贝
克隆方法:	限制性内切酶，多克隆位点
启动子:	lacZ
载体大小:	4989 bp
5' 测序引物及序列:	M13 Reverse:CAGGAAACAGCTATGAC
3' 测序引物及序列:	M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG
载体标签:	--
载体抗性:	氨苄青霉素
筛选标记:	TRP1
表达菌株:	酿酒酵母、白色念珠菌
备注:	--
稳定性:	稳表达
组成型/诱导型:	诱导型
病毒/非病毒:	非病毒

 

载体质粒图谱和多克隆位点信息

 

载体简介

 

载体序列

LOCUS       YEplac112	4989 bp 	DNA	SYN
DEFINITION  YEplac112
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="YEplac112"
                     /mol_type="other DNA"
     promoter        142..171
                     /label="lac_promoter"
     misc_feature    185..207
                     /label="M13_pUC_rev_primer"
     promoter        206..224
                     /label="M13_reverse_primer"
     misc_feature    287..442
                     /label="lacZ_a"
     promoter        complement(290..306)
                     /label="M13_forward20_primer"
     misc_feature    complement(299..321)
                     /label="M13_pUC_fwd_primer"
     rep_origin      complement(743..1907)
                     /label="2micron2_origin"
     gene            complement(2201..2875)
                     /label="TRP1"
                     /gene="TRP1"
     CDS             complement(2201..2875)
                     /label="ORF frame 3"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
                     KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
                     ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
                     SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
                     VKNAKK*"
     promoter        3118..3146
                     /label="AmpR_promoter"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
                     KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
                     ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
                     SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
                     VKNAKK*"
     gene            3188..4048
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
                     KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
                     ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
                     SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
                     VKNAKK*"
     CDS             3188..4048
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      4203..4822
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
   61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
  121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
  181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
  241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
  301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
  361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
  421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
  481 gcggtatttc acaccgcata tatcggatcg tacttgttac ccatcattga attttgaaca
  541 tccgaacctg ggagttttcc ctgaaacaga tagtatattt gaacctgtat aataatatat
  601 agtctagcgc tttacggaag acaatgtatg tatttcggtt cctggagaaa ctattgcatc
  661 tattgcatag gtaatcttgc acgtcgcatc cccggttcat tttctgcgtt tccatcttgc
  721 acttcaatag catatctttg ttaacgaagc atctgtgctt cattttgtag aacaaaaatg
  781 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa
  841 atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa
  901 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
  961 cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc
 1021 tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt
 1081 ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa
 1141 ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac
 1201 ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc
 1261 cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg
 1321 ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata
 1381 ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct
 1441 tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc
 1501 gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc
 1561 acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat
 1621 attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag
 1681 cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagctaga gaataggaac
 1741 ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag
 1801 ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat
 1861 atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc
 1921 tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg
 1981 ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa
 2041 ttggattagt ctcatccttc aatgctatca tttcctttga tattggatcg atccgatgat
 2101 aagctgtcaa acatgagaat tgatctttta tgcttgcttt tcaaaaggct tgcaggcaag
 2161 tgcacaaaca atacttaaat aaatactact cagtaataac ctatttctta gcatttttga
 2221 cgaaatttgc tattttgtta gagtctttta caccatttgt ctccacacct ccgcttacat
 2281 caacaccaat aacgccattt aatctaagcg catcaccaac attttctggc gtcagtccac
 2341 cagctaacat aaaatgtaag ctctcggggc tctcttgcct tccaacccag tcagaaatcg
 2401 agttccaatc caaaagttca cctgtcccac ctgcttctga atcaaacaag ggaataaacg
 2461 aatgaggttt ctgtgaagct gcactgagta gtatgttgca gtcttttgga aatacgagtc
 2521 ttttaataac tggcaaaccg aggaactctt ggtattcttg ccacgactca tctccatgca
 2581 gttggacgat atcaatgccg taatcattga ccagagccaa aacatcctcc ttaggttgat
 2641 tacgaaacac gccaaccaag tatttcggag tgcctgaact atttttatat gcttttacaa
 2701 gacttgaaat tttccttgca ataaccgggt caattgttct ctttctattg ggcacacata
 2761 taatacccag caagtcagca tcggaatcta gtgcacattc tgcggcctct gtgctctgca
 2821 agccgcaaac tttcaccaat ggaccagaac tacctgtgaa attaataaca gacatactcc
 2881 aagctgcctt tgtgtgctta atcacgtata ctcacgtgct caatagtcac caatgccctc
 2941 cctcttggcc ctctcctttt cttttttcga ccgaattaat tcttgaagac gaaagggcct
 3001 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
 3061 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
 3121 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
 3181 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
 3241 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
 3301 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
 3361 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
 3421 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
 3481 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
 3541 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
 3601 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
 3661 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
 3721 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
 3781 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
 3841 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
 3901 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
 3961 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
 4021 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
 4081 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
 4141 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
 4201 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
 4261 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
 4321 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
 4381 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
 4441 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
 4501 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
 4561 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
 4621 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
 4681 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
 4741 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
 4801 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
 4861 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
 4921 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
 4981 agcggaaga