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-20℃
6个月
pYC50
大量
钰博生物
1ug/5ug
出品人: | Ybscience |
---|---|
载体名称: | pYC50 |
质粒类型: | 酿酒酵母表达载体;Recipient integrative Vector;FLP/FRT系统载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | -- |
载体大小: | 6481 bp |
5' 测序引物及序列: | M13/pUC fwd primer 5’ CCAGTCACGACGTTGTAAAACG 3’ |
3' 测序引物及序列: | -- |
载体标签: | 13xMyc tag |
载体抗性: | 氨苄青霉素 |
筛选标记: | URA3,Nourseothricin |
克隆菌株: | DH10B感受态细胞 ,30°C |
宿主细胞(系): | 出芽酵母(酿酒酵母)、白色念珠菌 |
备注: | -- |
稳定性: | 瞬表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
酿酒酵母整合型载体还有:
pYC44
pYC56 红色荧光标签
pYC54 黄色荧光标签
pYC52 青色荧光标签
pYC50 13XMyc标签
pYC48 HA标签
pYC46 3XFlag标签
LOCUS pYC50 6481 bp DNA SYNDEFINITION pYC50ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors.FEATURES Location/Qualifiers source 1..6481 /organism="pYC50" /mol_type="other DNA" misc_feature complement(29..51) /label="pGEX_3_primer" promoter 196..409 /label="URA3_promoter" gene 411..1211 /label="URA3" /gene="URA3" CDS 411..1214 /label="ORF frame 3" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*" CDS complement(446..922) /label="ORF frame 1" /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*" misc_feature complement(1814..1962) /label="lacZ_a" /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*" misc_feature 1928..1950 /label="M13_pUC_fwd_primer" /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*" promoter 1943..1959 /label="M13_forward20_primer" /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*" promoter 1966..1984 /label="T7_promoter" /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*" misc_feature 2017..2033 /label="pBluescriptSK_primer" /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*" CDS complement(3287..3790) /label="ORF frame 1" /translation="MNSWSINIGNFQPQMTSTSFSGEFSSQTHQSSTNTMTSMFWSNF NIFNSQSSVPTRIRNNNETSQITIITISNESSRIWITIFTSIGFIIGFIIWENLSQWW INWNFSQSETITSSSNSKNSIGSERTIQSFNSFSITWNRSSVSISSIIQSSRHNCRID PVVYVRM*" CDS 3326..3898 /label="ORF frame 2" /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" promoter complement(4220..4239) /label="T3_promoter" /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" promoter complement(4256..4274) /label="M13_reverse_primer" /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" misc_feature complement(4273..4295) /label="M13_pUC_rev_primer" /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" rep_origin complement(4647..5266) /label="pBR322_origin" /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" gene complement(5421..6281) /label="Ampicillin" /gene="Ampicillin" /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" CDS complement(5421..6281) /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter complement(6323..6351) /label="AmpR_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*"ORIGIN 1 Tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc 241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca 301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat 361 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc ATGTCGAAAG 421 CTACATATAA GGAACGTGCT GCTACTCATC CTAGTCCTGT TGCTGCCAAG CTATTTAATA 481 TCATGCACGA AAAGCAAACA AACTTGTGTG CTTCATTGGA TGTTCGTACC ACCAAGGAAT 541 TACTGGAGTT AGTTGAAGCA TTAGGTCCCA AAATTTGTTT ACTAAAAACA CATGTGGATA 601 TCTTGACTGA TTTTTCCATG GAGGGCACAG TTAAGCCGCT AAAGGCATTA TCCGCCAAGT 661 ACAATTTTTT ACTCTTCGAA GACAGAAAAT TTGCTGACAT TGGTAATACA GTCAAATTGC 721 AGTACTCTGC GGGTGTATAC AGAATAGCAG AATGGGCAGA CATTACGAAT GCACACGGTG 781 TGGTGGGCCC AGGTATTGTT AGCGGTTTGA AGCAGGCGGC AGAAGAAGTA ACAAAGGAAC 841 CTAGAGGCCT TTTGATGTTA GCAGAATTGT CATGCAAGGG CTCCCTATCT ACTGGAGAAT 901 ATACTAAGGG TACTGTTGAC ATTGCGAAGA GCGACAAAGA TTTTGTTATC GGCTTTATTG 961 CTCAAAGAGA CATGGGTGGA AGAGATGAAG GTTACGATTG GTTGATTATG ACACCCGGTG 1021 TGGGTTTAGA TGACAAGGGA GACGCATTGG GTCAACAGTA TAGAACCGTG GATGATGTGG 1081 TCTCTACAGG ATCTGACATT ATTATTGTTG GAAGAGGACT ATTTGCAAAG GGAAGGGATG 1141 CTAAGGTAGA GGGTGAACGT TACAGAAAAG CAGGCTGGGA AGCATATTTG AGAAGATGCG 1201 GCCAGCAAAA CTAAaaaact gtattataag taaatgcatg tatactaaac tcacaaatta 1261 gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt 1321 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta 1381 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat 1441 aaatcaaaag aatagaccga gatagggttg agtgTTGTTC CAGTTTGGAA CAAGAGTCCA 1501 CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC 1561 CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA 1621 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG 1681 GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG 1741 GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG Cgccgctaca gggcgcgtcg 1801 cgCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG 1861 CTATTACGCC AGCTGGCGAA GGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA 1921 GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG AAttgtaata cgactcacta 1981 tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaactagtgg atcctagggT 2041 TAATTAACGG TGAACAAAAG CTAATCTCCG AGGAAGACTT GAACGGTGAA CAAAAATTAA 2101 TCTCAGAAGA AGACTTGAAC GGACTCGACG GTGAACAAAA GTTGATTTCT GAAGAAGATT 2161 TGAACGGTGA ACAAAAGCTA ATCTCCGAGG AAGACTTGAA CGGTGAACAA AAATTAATCT 2221 CAGAAGAAGA CTTGAACGGA CTCGACGGTG AACAAAAGTT GATTTCTGAA GAAGATTTGA 2281 ACGGTGAACA AAAGCTAATC TCCGAGGAAG ACTTGAACGG TGAACAAAAA TTAATCTCAG 2341 AAGAAGACTT GAACGGACTC GACGGTGAAC AAAAGTTGAT TTCTGAAGAA GATTTGAACG 2401 GTGAACAAAA GCTAATCTCC GAGGAAGACT TGAACGGTGA ACAAAAATTA ATCTCAGAAG 2461 AAGACTTGAA CGGACTCGAC GGTGAACAAA AGTTGATTTC TGAAGAAGAT TTGAACGGTG 2521 AACAAAAGCT AATCTCCGAG GAAGACTTGA ACGGTGAACA AAAATTAATC AATCACtagc 2581 tagtgaattc gcgccagatc cGAAGTTCCT ATACTTTCTA GAGAATAGGA ACTTCgtgcg 2641 cttttgaacc acgtaaagtg ctgtaaaaca gctctattca atgaggtaaa tgataaagtt 2701 tataagagac caaaaaattc aaaaaaaaaa ttgaaaaatt atattggtat ttatatgaaa 2761 caatagttca gttcaaagcg ctagcttttc tcatctcaac taaccggtat taggtgggta 2821 tttatttaat atgggaacaa gaaatgtgac actaatgttc atgtttttat tttcattatt 2881 tattctatat ggtttgggac ttgatgttat gtcttaattc atagaGATCC gcttgcctcg 2941 tccccgccgg gtcacccggc cagcgacatg gaggcccaga ataccctcct tgacagtctt 3001 gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc catacatccc 3061 catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag caaaaattac 3121 ggctcctcgc tgcagacctg cgagcaggga aacgctcccc tcacagacgc gttgaattgt 3181 ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact gaggttcttc 3241 tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac atccgaacat 3301 aaacaaccgG ATCgatccta caattATGTC TACTACTTTG GATGATACTG CTTATAGATA 3361 CAGAACTTCT GTTCCAGGTG ATGCTGAAGC TATTGAAGCT TTGGATGGTT CTTTCACTAC 3421 CGATACTGTT TTTAGAGTTA CTGCTACTGG TGATGGTTTC ACTTTGAGAG AAGTTCCAGT 3481 TGATCCACCA TTGACTAAGG TTTTCCCAGA TGATGAATCC GATGATGAAT CCGATGCTGG 3541 TGAAGATGGT GATCCAGATT CTAGAACTTT CGTTGCTTAT GGTGATGATG GTGATTTGGC 3601 TGGTTTCGTT GTTGTTTCTT ATTCTGGTTG GAACAGAAGA TTGACTGTTG AAGATATTGA 3661 AGTTGCTCCA GAACATAGAG GTCATGGTGT TGGTAGAGCT TTGATGGGTT TGGCTACTGA 3721 ATTCGCCAGA GAAAGAGGTG CTGGTCATTT GTGGTTGGAA GTTACCAATG TTAATGCTCC 3781 AGCTATTCAT GCTTATAGAA GAATGGGTTT CACTTTGTGT GGTTTGGATA CTGCTTTATA 3841 CGATGGTACT GCTTCCGATG GTGAACAAGC TTTGTATATG TCTATGCCAT GTCCATAAta 3901 acTCGACcag tactgacaat aaaaagattc ttgttttcaa gaacttgtca tttgtatagt 3961 ttttttatat tgtagttgtt ctattttaat caaatgttag cgtgatttat attttttttc 4021 gcctcgacat catctgccca gatgcgaagt taagtgcgca gaaagtaata tcatgcgtca 4081 atcgtatgtg aatgctggtc gctatactgc tgtcgattcg atactaacgc cgccatccag 4141 tgTCGAtcga cGAAGTTCCT ATACTTTCTA GAGAATAGGA ACTTCGTCct cgaggggggg 4201 cccggtacca gcttttgttc cctttagtga gggttaattc cgagcttggc gtaatcatgg 4261 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa cataggagcc 4321 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga ggtaactcac attaattgcg 4381 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 4441 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 4501 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 4561 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 4621 caaaaggcca ggaaccgtaa aaaggcCGCG TTGCTGGCGT TTTTCCATAG GCTCGGCCCC 4681 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA 4741 TAAAGATACC AGGCGTTCCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG 4801 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCAATGC 4861 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC 4921 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC 4981 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG 5041 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA 5101 AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT 5161 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG 5221 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTtttc tacggggtct 5281 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 5341 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 5401 gagtaaactt ggtctgacag TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC 5461 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTGCCCGTCG TGTAGATAAC TACGATACGG 5521 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT 5581 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA 5641 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG 5701 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG 5761 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC 5821 CCCATGTTGT GAAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG 5881 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG 5941 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG 6001 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT 6061 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG 6121 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA 6181 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA 6241 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA Tactcttcct ttttcaatat 6301 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 6361 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa 6421 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt 6481 c
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