- ¥800 - 1200
- Ybscience
- 中国/美国
- YB-0552
- 2025年07月16日
14 年金牌会员
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pGL3-control
- 库存：
大量
- 供应商：
钰博生物
- 规格：
1ug/5ug
pGL3-control
载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 载体名称: | pGL3-control |
| 质粒类型: | 哺乳细胞表达；启动子增强子报告系统 |
| 高拷贝/低拷贝: | -- |
| 启动子: | SV40 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5256bp |
| 5' 测序引物及序列: | RVprimer3 Fwd: CTAGCAAAATAGGCTGTCCC |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素（Ampicillin） |
| 筛选标记: | -- |
| 备注: | pGL3-control是荧光素酶报告载体，含有SV40 增强子和启动子，增强荧光素酶的表达。 |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | 非病毒 |
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGL3-Control 5256 bp DNA SYN
DEFINITION pGL3-Control
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..5256
/organism="pGL3-Control"
/mol_type="other DNA"
promoter 48..250
/label="SV40_promoter"
rep_origin 95..172
/label="SV40_origin"
misc_feature 157..176
/label="SV40pro_F_primer"
gene 280..1932
/label="firefly_Luciferase"
/gene="firefly_Luciferase"
CDS 280..1932
/label="ORF frame 1"
misc_feature complement(346..364)
/label="LucNrev_primer"
misc_feature complement(1984..2003)
/label="EBV_rev_primer"
misc_feature 2209..2436
/label="SV40_enhancer"
misc_feature 2416..2436
/label="pBABE_3_primer"
rep_origin complement(2744..3363)
/label="pBR322_origin"
gene complement(3518..4378)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(3518..4378)
/label="ORF frame 3"
promoter complement(4420..4448)
/label="AmpR_promoter"
rep_origin 4527..4833
/label="f1_origin"
misc_feature 5096..5249
/label="TB"
terminator 5096..5144
/label="synth_PA_terminator"
terminator 5096..5249
/label="up_PA_terminator"
misc_feature 5198..5217
/label="RVprimer3_primer"
ORIGIN
1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta
61 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
121 cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc
181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
241 caaaaagctt ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata
301 aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat
361 aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc
421 gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg
481 aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa
541 ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac
601 atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc
661 gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa
721 aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc
781 gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat
841 agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt
901 gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt
961 ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt
1021 ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga
1081 tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg
1141 gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct
1201 aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt
1261 gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca
1321 gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca
1381 ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga
1441 ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg
1501 accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac
1561 gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat
1621 caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca
1681 ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg
1741 gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca
1801 accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc
1861 ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag
1921 atcgccgtgt aattctagag tcggggcggc cggccgcttc gagcagacat gataagatac
1981 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
2041 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
2101 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
2161 aagtaaaacc tctacaaatg tggtaaaatc gataaggatc tgaacgatgg agcggagaat
2221 gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg
2281 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
2341 cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
2401 ctggggactt tccacaccct aactgacaca cattccacag cggatccgtc gaccgatgcc
2461 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc
2521 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt
2581 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
2641 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
2701 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
2761 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
2821 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
2881 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
2941 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
3001 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
3061 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
3121 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
3181 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
3241 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
3301 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
3361 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
3421 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
3481 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
3541 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
3601 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
3661 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
3721 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
3781 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
3841 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
3901 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
3961 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
4021 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
4081 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
4141 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
4201 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
4261 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
4321 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
4381 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
4441 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
4501 tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
4561 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
4621 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
4681 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
4741 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
4801 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
4861 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
4921 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttcccat tcgccattca
4981 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagccca
5041 agctaccatg ataagtaagt aatattaagg tacgggaggt acttggagcg gccgcaataa
5101 aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac
5161 atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag
5221 tgcaagtgca ggtgccagaa catttctcta tcgata
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
Short-Fragment DNA-Mediated In Vivo DNA Electroporation Delivery
) for facilitating electroporation-mediated gene transfer. The plasmid pGL3-control encoding firefly luciferase was injected into tissue together with or without sf-DNA in different length or dose. Immediately after injection, the tissues were electroporated
Transfection of Primary Cultures of Rat Hepatocytes
of male rat hepatocytes. Hepatocytes were isolated by the collagenase perfusion method and then cultured on Matrigel-coated plates for 24 h before transfection. The cells were transfected with either pGL3-Control or pGL3-Basic plasmids. The efficiency
Transient Transfection and Adrenergic Receptor Promoter Analysis
, IN) and Lipofectin (Gibco-BRL, Grand Island, NY). Cardiac myocytes were prepared from 3-d-old neonatal rats. Cells were transfected with pGL3-control vector 2 d after plating in 24-well plates to test efficiency on primary culture. The DNA doses are indicated
技术资料暂无技术资料 索取技术资料
pGL3-control
¥800 - 1200
